Expression and purification of nonglycosylated SIV proteins, and their use in induction and detection of SIV-specific immune responses.
Two commercially available expression vectors were modified to generate plasmids pGEXcPk and pQ9cPk. Proteins expressed from pGEXcPk and pQ9cPk had a short oligopeptide tag termed Pk at their carboxy termini and either glutathione S-transferase (GST) or a small histidine (His) tag, respectively, at...
| Main Authors: | , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
1994
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