Expression and purification of nonglycosylated SIV proteins, and their use in induction and detection of SIV-specific immune responses.
Two commercially available expression vectors were modified to generate plasmids pGEXcPk and pQ9cPk. Proteins expressed from pGEXcPk and pQ9cPk had a short oligopeptide tag termed Pk at their carboxy termini and either glutathione S-transferase (GST) or a small histidine (His) tag, respectively, at...
| Main Authors: | , , , , , |
|---|---|
| Format: | Journal article |
| Jezik: | English |
| Izdano: |
1994
|