Pan-cancer analysis of tissue and single-cell HIF-pathway activation using a conserved gene signature
Activation of cellular hypoxia pathways, orchestrated by HIF (hypoxia-inducible factor) transcription factors, is a common feature of multiple tumor types, resulting from microenvironment factors and oncogenic mutation. Although they help drive many of the “hallmarks” of cancer and are associated wi...
| Main Authors: | , , , , , |
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| Format: | Journal article |
| Language: | English |
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Elsevier
2022
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| _version_ | 1797108713321922560 |
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| author | Lombardi, O Li, R Halim, S Choudhry, H Ratcliffe, PJ Mole, DR |
| author_facet | Lombardi, O Li, R Halim, S Choudhry, H Ratcliffe, PJ Mole, DR |
| author_sort | Lombardi, O |
| collection | OXFORD |
| description | Activation of cellular hypoxia pathways, orchestrated by HIF (hypoxia-inducible factor) transcription factors, is a common feature of multiple tumor types, resulting from microenvironment factors and oncogenic mutation. Although they help drive many of the “hallmarks” of cancer and are associated with poor outcome and resistance to therapy, the transcriptional targets of HIF vary considerably depending on the cell type. By integrating 72 genome-wide assays of HIF binding and transcriptional regulation from multiple cancer types, we define a consensus set of 48 HIF target genes that is highly conserved across cancer types and cell lineages. These genes provide an effective marker of HIF activation in bulk and single-cell transcriptomic analyses across a wide range of cancer types and in malignant and stromal cell types. This allows the tissue-orchestrated responses to the hypoxic tumor microenvironment and to oncogenic HIF activation to be deconvoluted at the tumor and single-cell level. |
| first_indexed | 2024-03-07T07:32:32Z |
| format | Journal article |
| id | oxford-uuid:d19f6882-38af-427f-8a9a-29a0057bf6dc |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T07:32:32Z |
| publishDate | 2022 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | oxford-uuid:d19f6882-38af-427f-8a9a-29a0057bf6dc2023-01-20T15:33:43ZPan-cancer analysis of tissue and single-cell HIF-pathway activation using a conserved gene signatureJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:d19f6882-38af-427f-8a9a-29a0057bf6dcEnglishSymplectic ElementsElsevier2022Lombardi, OLi, RHalim, SChoudhry, HRatcliffe, PJMole, DRActivation of cellular hypoxia pathways, orchestrated by HIF (hypoxia-inducible factor) transcription factors, is a common feature of multiple tumor types, resulting from microenvironment factors and oncogenic mutation. Although they help drive many of the “hallmarks” of cancer and are associated with poor outcome and resistance to therapy, the transcriptional targets of HIF vary considerably depending on the cell type. By integrating 72 genome-wide assays of HIF binding and transcriptional regulation from multiple cancer types, we define a consensus set of 48 HIF target genes that is highly conserved across cancer types and cell lineages. These genes provide an effective marker of HIF activation in bulk and single-cell transcriptomic analyses across a wide range of cancer types and in malignant and stromal cell types. This allows the tissue-orchestrated responses to the hypoxic tumor microenvironment and to oncogenic HIF activation to be deconvoluted at the tumor and single-cell level. |
| spellingShingle | Lombardi, O Li, R Halim, S Choudhry, H Ratcliffe, PJ Mole, DR Pan-cancer analysis of tissue and single-cell HIF-pathway activation using a conserved gene signature |
| title | Pan-cancer analysis of tissue and single-cell HIF-pathway activation using a conserved gene signature |
| title_full | Pan-cancer analysis of tissue and single-cell HIF-pathway activation using a conserved gene signature |
| title_fullStr | Pan-cancer analysis of tissue and single-cell HIF-pathway activation using a conserved gene signature |
| title_full_unstemmed | Pan-cancer analysis of tissue and single-cell HIF-pathway activation using a conserved gene signature |
| title_short | Pan-cancer analysis of tissue and single-cell HIF-pathway activation using a conserved gene signature |
| title_sort | pan cancer analysis of tissue and single cell hif pathway activation using a conserved gene signature |
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