Fluorescence nanoscopy in whole cells by asynchronous localization of photoswitching emitters.

We demonstrate nanoscale resolution in far-field fluorescence microscopy using reversible photoswitching and localization of individual fluorophores at comparatively fast recording speeds and from the interior of intact cells. These advancements have become possible by asynchronously recording the p...

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Bibliographic Details
Main Authors: Egner, A, Geisler, C, von Middendorff, C, Bock, H, Wenzel, D, Medda, R, Andresen, M, Stiel, A, Jakobs, S, Eggeling, C, Schönle, A, Hell, S
Format: Journal article
Language:English
Published: 2007