A modified density gradient proteomic-based method to analyze endolysosomal proteins in cardiac tissue

The importance of lysosomes in cardiac physiology and pathology is well established, and evidence for roles in calcium signaling is emerging. We describe a label-free proteomics method suitable for small cardiac tissue biopsies based on density-separated fractionation, which allows study of endolyso...

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Bibliographic Details
Main Authors: Ayagama, T, Bose, SJ, Capel, RA, Priestman, DA, Berridge, G, Fischer, R, Galione, A, Platt, FM, Kramer, H, Burton, RAB
Format: Journal article
Language:English
Published: Elsevier 2021