Crystallization and preliminary X-ray diffraction studies on recombinant isopenicillin N synthase from Aspergillus nidulans.

Recombinant Aspergillus nidulans isopenicillin N synthase was purified from an Escherichia coli expression system. The apoenzyme in the presence of saturating concentrations of MnCl2 could be crystallized by either macro- or microseeding, using the hanging drop vapor diffusion technique with polyeth...

Täydet tiedot

Bibliografiset tiedot
Päätekijät: Roach, P, Schofield, C, Baldwin, J, Clifton, I, Hajdu, J
Aineistotyyppi: Journal article
Kieli:English
Julkaistu: 1995