Crystallization and preliminary X-ray diffraction studies on recombinant isopenicillin N synthase from Aspergillus nidulans.

Recombinant Aspergillus nidulans isopenicillin N synthase was purified from an Escherichia coli expression system. The apoenzyme in the presence of saturating concentrations of MnCl2 could be crystallized by either macro- or microseeding, using the hanging drop vapor diffusion technique with polyeth...

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Bibliografische gegevens
Hoofdauteurs: Roach, P, Schofield, C, Baldwin, J, Clifton, I, Hajdu, J
Formaat: Journal article
Taal:English
Gepubliceerd in: 1995