Crystallization and preliminary X-ray diffraction studies on recombinant isopenicillin N synthase from Aspergillus nidulans.

Recombinant Aspergillus nidulans isopenicillin N synthase was purified from an Escherichia coli expression system. The apoenzyme in the presence of saturating concentrations of MnCl2 could be crystallized by either macro- or microseeding, using the hanging drop vapor diffusion technique with polyeth...

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Những tác giả chính: Roach, P, Schofield, C, Baldwin, J, Clifton, I, Hajdu, J
Định dạng: Journal article
Ngôn ngữ:English
Được phát hành: 1995

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