Crystallization and preliminary X-ray diffraction studies on recombinant isopenicillin N synthase from Aspergillus nidulans.
Recombinant Aspergillus nidulans isopenicillin N synthase was purified from an Escherichia coli expression system. The apoenzyme in the presence of saturating concentrations of MnCl2 could be crystallized by either macro- or microseeding, using the hanging drop vapor diffusion technique with polyeth...
Những tác giả chính: | Roach, P, Schofield, C, Baldwin, J, Clifton, I, Hajdu, J |
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Định dạng: | Journal article |
Ngôn ngữ: | English |
Được phát hành: |
1995
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