$Crystallization and preliminary X-ray diffraction studies on recombinant isopenicillin N synthase from Aspergillus nidulans.$

Crystallization and preliminary X-ray diffraction studies on recombinant isopenicillin N synthase from Aspergillus nidulans.

Recombinant Aspergillus nidulans isopenicillin N synthase was purified from an Escherichia coli expression system. The apoenzyme in the presence of saturating concentrations of MnCl2 could be crystallized by either macro- or microseeding, using the hanging drop vapor diffusion technique with polyeth...

Disgrifiad llawn

Manylion Llyfryddiaeth
Prif Awduron:	Roach, P, Schofield, C, Baldwin, J, Clifton, I, Hajdu, J
Fformat:	Journal article
Iaith:	English
Cyhoeddwyd:	1995

Eitemau Tebyg

Glutamine-330 is not essential for activity in isopenicillin N synthase from Aspergillus nidulans.
gan: Sami, M, et al.
Cyhoeddwyd: (1997)

Crystallization and preliminary X-ray diffraction studies on a recombinant isopenicillin N synthase from Cephalosporium acremonium.
gan: Fujishima, Y, et al.
Cyhoeddwyd: (1994)

Structure of isopenicillin N synthase complexed with substrate and the mechanism of penicillin formation.
gan: Roach, P, et al.
Cyhoeddwyd: (1997)

Crystal structure of isopenicillin N synthase is the first from a new structural family of enzymes.
gan: Roach, P, et al.
Cyhoeddwyd: (1995)

Anaerobic crystallisation of an isopenicillin N synthase.Fe(II).substrate complex demonstrated by X-ray studies.
gan: Roach, P, et al.
Cyhoeddwyd: (1996)

EXAFS STUDIES OF ISOPENICILLIN-N SYNTHASE
gan: Randall, C, et al.
Cyhoeddwyd: (1992)

Anaerobic crystallisation of an isopenicillin N synthase center dot Fe(II)center dot substrate complex demonstrated by X-ray studies
gan: Roach, P, et al.
Cyhoeddwyd: (1996)

The crystal structure of isopenicillin N synthase with a dipeptide substrate analogue.
gan: Daruzzaman, A, et al.
Cyhoeddwyd: (2013)

ISOPENICILLIN-N SYNTHASE - A NEW MODE OF REACTIVITY
gan: Baldwin, J, et al.
Cyhoeddwyd: (1991)

ISOPENICILLIN-N SYNTHASE - A NEW MODE OF REACTIVITY
gan: Baldwin, J, et al.
Cyhoeddwyd: (1992)

The crystal structure of an LLL-configured depsipeptide substrate analogue bound to isopenicillin N synthase.
gan: Ge, W, et al.
Cyhoeddwyd: (2010)

Structural studies on the reaction of isopenicillin N synthase with the substrate analogue delta-(l-alpha-aminoadipoyl)-l-cysteinyl-d-alpha-aminobutyrate.
gan: Long, A, et al.
Cyhoeddwyd: (2003)

FORMATION OF A NOVEL BICYCLIC GAMMA-LACTAM WITH ISOPENICILLIN-N SYNTHASE
gan: Baldwin, J, et al.
Cyhoeddwyd: (1989)

Identification of many crystal forms of Aspergillus nidulans dehydroquinate synthase.
gan: Nichols, C, et al.
Cyhoeddwyd: (2001)

GAMMA-LACTAM FORMATION FROM TRIPEPTIDES WITH ISOPENICILLIN-N SYNTHASE
gan: Baldwin, J, et al.
Cyhoeddwyd: (1988)

Analysis of the conversion of delta-(L-alpha-aminoadipoyl)-L-cysteinyl-D-alpha-aminobutyrate by active-site mutants of Aspergillus nidulans isopenicillin N synthase.
gan: Rowe, C, et al.
Cyhoeddwyd: (1998)

Molecular weight analysis of isopenicillin N synthase by electrospray mass spectrometry.
gan: Aplin, RT, et al.
Cyhoeddwyd: (1990)

X-ray absorption studies of the ferrous active site of isopenicillin N synthase and related model complexes.
gan: Randall, C, et al.
Cyhoeddwyd: (1993)

Studies on side chain interactions during the isopenicillin N synthase catalysed biosynthesis of penicillins
gan: Cavelier, F, et al.
Cyhoeddwyd: (1997)

Purification, crystallization and preliminary X-ray diffraction of anthocyanidin synthase from Arabidopsis thaliana.
gan: Turnbull, J, et al.
Cyhoeddwyd: (2001)

Studies of isopenicillin N synthase enzymatic properties using a continuous spectrophotometric assay.
gan: Dubus, A, et al.
Cyhoeddwyd: (2000)

Structural studies on the reaction of isopenicillin N synthase with the truncated substrate analogues delta-(L-alpha-aminoadipoyl)-L-cysteinyl-glycine and delta-(L-alpha-aminoadipoyl)-L-cysteinyl-D-alanine.
gan: Long, A, et al.
Cyhoeddwyd: (2005)

The crystal structure of isopenicillin N synthase with δ-((L)-α-aminoadipoyl)-(L)-cysteinyl-(D)-methionine reveals thioether coordination to iron.
gan: Clifton, I, et al.
Cyhoeddwyd: (2011)

Crystallographic studies on the binding of selectively deuterated LLD- and LLL-substrate epimers by isopenicillin N synthase.
gan: Ge, W, et al.
Cyhoeddwyd: (2010)

Mechanistic studies on isopenicillin n synthase
gan: Abbott, S
Cyhoeddwyd: (1991)

Structural studies on the reaction of isopenicillin N synthase with a sterically demanding depsipeptide substrate analogue.
gan: Ge, W, et al.
Cyhoeddwyd: (2009)

Roles of 2-oxoglutarate oxygenases and isopenicillin N synthase in ß-lactam biosynthesis
gan: Rabe, P, et al.
Cyhoeddwyd: (2018)

Mitotic recombination accelerates adaptation in the fungus Aspergillus nidulans.
gan: Sijmen E Schoustra, et al.
Cyhoeddwyd: (2007-04-01)

X-ray free-electron laser studies reveal correlated motion during isopenicillin N synthase catalysis
gan: Rabe, P, et al.
Cyhoeddwyd: (2021)

Modification of iron binding ligands in isopenicillin n synthase
gan: Sami, M, et al.
Cyhoeddwyd: (1998)

Investigations into the post-translational modification and mechanism of isopenicillin N:acyl-CoA acyltransferase using electrospray mass spectrometry.
gan: Aplin, RT, et al.
Cyhoeddwyd: (1993)

Isopenicillin N synthase binds δ-(L-α-aminoadipoyl)-L-cysteinyl-D-thia-allo-isoleucine through both sulfur atoms.
gan: Clifton, I, et al.
Cyhoeddwyd: (2011)

The interaction of isopenicillin N synthase with homologated substrate analogues δ-(L-α-aminoadipoyl)-L-homocysteinyl-D-Xaa characterised by protein crystallography.
gan: Daruzzaman, A, et al.
Cyhoeddwyd: (2013)

Asexual Recombination in a uvsH Mutant of Aspergillus nidulans
gan: S A SOUZA-JÚNIOR, et al.
Cyhoeddwyd: (2007-01-01)

Isopenicillin N synthase mediates thiolate oxidation to sulfenate in a depsipeptide substrate analogue: implications for oxygen binding and a link to nitrile hydratase?
gan: Ge, W, et al.
Cyhoeddwyd: (2008)

Spectroscopic studies reveal details of substrate-induced conformational changes distant from the active site in isopenicillin N synthase
gan: Rabe, P, et al.
Cyhoeddwyd: (2022)

Photoaffinity labelling of isopenicillin N synthetase.
gan: Baldwin, J, et al.
Cyhoeddwyd: (1990)

Increase in mitotic recombination in diploid cells of Aspergillus nidulans in response to ethidium bromide
gan: Tânia C.A. Becker, et al.
Cyhoeddwyd: (2003-01-01)

Ligand-induced conformational changes and a mechanism for domain closure in Aspergillus nidulans dehydroquinate synthase.
gan: Nichols, C, et al.
Cyhoeddwyd: (2003)

Acyl coenzyme A: 6-aminopenicillanic acid acyltransferase from Penicillium chrysogenum and Aspergillus nidulans.
gan: Whiteman, P, et al.
Cyhoeddwyd: (1990)