19F NMR studies of the native and denatured states of green fluorescent protein.
Biosynthetic preparation and (19)F NMR experiments on uniformly 3-fluorotyrosine-labeled green fluorescent protein (GFP) are described. The (19)F NMR signals of all 10 fluorotyrosines are resolved in the protein spectrum with signals spread over 10 ppm. Each tyrosine in GFP was mutated in turn to ph...
| Main Authors: | , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
2006
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