Transgenic microRNA inhibition with spatiotemporal specificity in intact organisms.
MicroRNAs are important regulators of gene expression, yet the functional outputs of most microRNA-target interactions remain elusive. Here we introduce the Drosophila melanogaster microRNA sponge (miR-SP) as a powerful transgenic technology to dissect the function of every microRNA with precise spa...
| Main Authors: | , , , |
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| Format: | Journal article |
| Language: | English |
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2009
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| _version_ | 1797097311282659328 |
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| author | Loya, C Lu, C Van Vactor, D Fulga, T |
| author_facet | Loya, C Lu, C Van Vactor, D Fulga, T |
| author_sort | Loya, C |
| collection | OXFORD |
| description | MicroRNAs are important regulators of gene expression, yet the functional outputs of most microRNA-target interactions remain elusive. Here we introduce the Drosophila melanogaster microRNA sponge (miR-SP) as a powerful transgenic technology to dissect the function of every microRNA with precise spatiotemporal resolution. miR-SPs can be used to characterize tissue-specific microRNA loss-of-function phenotypes, define the spatial regulation of their effectors and uncover interactions between microRNAs and other genes. Using themiR-SP system, we identified an essential role of the conserved microRNA miR-8, in neuromuscular junction formation. Tissue-specific silencing revealed that postsynaptic activity of miR-8 is important for normal neuromuscular junction morphogenesis. Given that miR-SPs rely on a bipartite modular expression system, they could be used to elucidate the endogenous function of microRNAs in any species in which conditional expression can be achieved. |
| first_indexed | 2024-03-07T04:53:43Z |
| format | Journal article |
| id | oxford-uuid:d5dbaae9-026e-4d59-8b05-cf12bbf376ef |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T04:53:43Z |
| publishDate | 2009 |
| record_format | dspace |
| spelling | oxford-uuid:d5dbaae9-026e-4d59-8b05-cf12bbf376ef2022-03-27T08:29:01ZTransgenic microRNA inhibition with spatiotemporal specificity in intact organisms.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:d5dbaae9-026e-4d59-8b05-cf12bbf376efEnglishSymplectic Elements at Oxford2009Loya, CLu, CVan Vactor, DFulga, TMicroRNAs are important regulators of gene expression, yet the functional outputs of most microRNA-target interactions remain elusive. Here we introduce the Drosophila melanogaster microRNA sponge (miR-SP) as a powerful transgenic technology to dissect the function of every microRNA with precise spatiotemporal resolution. miR-SPs can be used to characterize tissue-specific microRNA loss-of-function phenotypes, define the spatial regulation of their effectors and uncover interactions between microRNAs and other genes. Using themiR-SP system, we identified an essential role of the conserved microRNA miR-8, in neuromuscular junction formation. Tissue-specific silencing revealed that postsynaptic activity of miR-8 is important for normal neuromuscular junction morphogenesis. Given that miR-SPs rely on a bipartite modular expression system, they could be used to elucidate the endogenous function of microRNAs in any species in which conditional expression can be achieved. |
| spellingShingle | Loya, C Lu, C Van Vactor, D Fulga, T Transgenic microRNA inhibition with spatiotemporal specificity in intact organisms. |
| title | Transgenic microRNA inhibition with spatiotemporal specificity in intact organisms. |
| title_full | Transgenic microRNA inhibition with spatiotemporal specificity in intact organisms. |
| title_fullStr | Transgenic microRNA inhibition with spatiotemporal specificity in intact organisms. |
| title_full_unstemmed | Transgenic microRNA inhibition with spatiotemporal specificity in intact organisms. |
| title_short | Transgenic microRNA inhibition with spatiotemporal specificity in intact organisms. |
| title_sort | transgenic microrna inhibition with spatiotemporal specificity in intact organisms |
| work_keys_str_mv | AT loyac transgenicmicrornainhibitionwithspatiotemporalspecificityinintactorganisms AT luc transgenicmicrornainhibitionwithspatiotemporalspecificityinintactorganisms AT vanvactord transgenicmicrornainhibitionwithspatiotemporalspecificityinintactorganisms AT fulgat transgenicmicrornainhibitionwithspatiotemporalspecificityinintactorganisms |