A new dark quencher for use in genetic analysis.
A novel, long-wavelength, non-fluorescent quencher (LQ), based on 1,4-diaminoanthraquinone, has been incorporated at the 3' and 5'-termini of oligonucleotides. The quencher has been used in Molecular beacons, efficiently quenching the long wavelength fluorophore, Cy5.
| Main Authors: | , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
2003
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