Enzymatic incorporation and fluorescent labelling of cyclooctyne-modified deoxyuridine triphosphates in DNA.

Enzymatic incorporation and fluorescent labelling of cyclooctyne-modified deoxyuridine triphosphates in DNA.

The amino group of 5-aminopropargyl-2'-deoxyuridine-5'-triphosphate was labelled with dibenzocyclooctyne (DIBO) and two derivatives of bicyclo [6.1.0] non-4-yne (BCN) with short and long linkers to produce three different cycloalkyne-modified deoxyuridine triphosphates. BCN was successfull...

Ausführliche Beschreibung

Bibliographische Detailangaben
Hauptverfasser:	Ren, X, Gerowska, M, El-Sagheer, A, Brown, T
Format:	Journal article
Sprache:	English
Veröffentlicht:	Elsevier 2014

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