Enzymatic incorporation and fluorescent labelling of cyclooctyne-modified deoxyuridine triphosphates in DNA.

Enzymatic incorporation and fluorescent labelling of cyclooctyne-modified deoxyuridine triphosphates in DNA.

The amino group of 5-aminopropargyl-2'-deoxyuridine-5'-triphosphate was labelled with dibenzocyclooctyne (DIBO) and two derivatives of bicyclo [6.1.0] non-4-yne (BCN) with short and long linkers to produce three different cycloalkyne-modified deoxyuridine triphosphates. BCN was successfull...

Volledige beschrijving

Bibliografische gegevens
Hoofdauteurs:	Ren, X, Gerowska, M, El-Sagheer, A, Brown, T
Formaat:	Journal article
Taal:	English
Gepubliceerd in:	Elsevier 2014

Gelijkaardige items

Synthesis and Enzymatic Incorporation of Modified Deoxyuridine Triphosphates
door: Erkai Liu, et al.
Gepubliceerd in: (2015-07-01)

Azide and trans-cyclooctene dUTPs: incorporation into DNA probes and fluorescent click-labelling.
door: Ren, X, et al.
Gepubliceerd in: (2015)

Synthesis of Polyanionic C5-Modified 2′-Deoxyuridine and 2′-Deoxycytidine-5′-Triphosphates and Their Properties as Substrates for DNA Polymerases
door: Claire Dutson, et al.
Gepubliceerd in: (2021-04-01)

Efficient enzymatic synthesis and dual-colour fluorescent labelling of DNA probes using long chain azido-dUTP and BCN dyes.
door: Ren, X, et al.
Gepubliceerd in: (2016)

Structural dynamics of human deoxyuridine 5’-triphosphate nucleotidohydrolase (dUTPase)
door: Ravdna Sarre, et al.
Gepubliceerd in: (2024-10-01)

Site-Selective Cysteine-Cyclooctyne Conjugation
door: Zhang, Chi, et al.
Gepubliceerd in: (2020)

Deoxyuridine triphosphates modified with tyrosine aromatic groups for direct electrochemical detection of double-stranded DNA products of isothermal recombinase polymerase amplification
door: Elena V. Suprun, et al.
Gepubliceerd in: (2021-10-01)

Crystallization and preliminary crystallographic analysis of deoxyuridine 5'-triphosphate nucleotidohydrolase from Bacillus subtilis.
door: Persson, R, et al.
Gepubliceerd in: (2001)

Screening and Identification of a Novel Anti-tuberculosis Compound That Targets Deoxyuridine 5′-Triphosphate Nucleotidohydrolase
door: Yu Zhang, et al.
Gepubliceerd in: (2021-10-01)

Monitoring DNA replication in fission yeast by incorporation of 5-ethynyl-2'-deoxyuridine
door: Hua, H, et al.
Gepubliceerd in: (2011)

Monitoring DNA replication in fission yeast by incorporation of 5-ethynyl-2'-deoxyuridine.
door: Hua, H, et al.
Gepubliceerd in: (2011)

DNA triplex formation with 5-dimethylaminopropargyl deoxyuridine.
door: Rusling, D, et al.
Gepubliceerd in: (2009)

Intramolecular Nicholas Reaction Enables the Stereoselective Synthesis of Strained Cyclooctynes
door: Diego M. Monzón, et al.
Gepubliceerd in: (2021-03-01)

Base-Resolution Analysis of Deoxyuridine at the Genome Scale Based on the Artificial Incorporation Modified Nucleobase
door: Yafen Wang, et al.
Gepubliceerd in: (2021-04-01)

Simultaneous visualization of FISH signals and bromo-deoxyuridine incorporation by formamide-free DNA denaturation.
door: Moralli, D, et al.
Gepubliceerd in: (2010)

A Mild Aqueous Sonogashira Reaction as a Fluorescent Labeling Strategy for 5-Bromide-2′-Deoxyuridine
door: Shufang Wang, et al.
Gepubliceerd in: (2018-01-01)

[3+2]-Cycloaddition of 2-(tert-butylsulfanyl)propenoic acid derivatives to cyclooctyne
door: Dietrich Döpp, et al.
Gepubliceerd in: (2001-11-01)

Synthesis and Photophysical Study of 2′-Deoxyuridines Labeled with Fluorene Derivatives
door: Gil Tae Hwang, et al.
Gepubliceerd in: (2012-10-01)

Incorporation of 5-Bromo-2′-deoxyuridine into DNA and Proliferative Behavior of Cerebellar Neuroblasts: All That Glitters Is Not Gold
door: Joaquín Martí-Clúa
Gepubliceerd in: (2021-06-01)

Investigation of the substrate properties of fluorescently labeled pyrimidine triphosphates in recombinase polymerase amplification
door: A. S. Epifanov, et al.
Gepubliceerd in: (2024-11-01)

Atomic scissors: a new method of tracking the 5-bromo-2'-deoxyuridine-labeled DNA in situ.
door: Anna Ligasová, et al.
Gepubliceerd in: (2012-01-01)

A highly fluorescent DNA toolkit: synthesis and properties of oligonucleotides containing new Cy3, Cy5 and Cy3B monomers.
door: Hall, L, et al.
Gepubliceerd in: (2012)

Detection of S-phase cell cycle progression using 5-ethynyl-2′-deoxyuridine incorporation with click chemistry, an alternative to using 5-bromo-2′-deoxyuridine antibodies
door: Suzanne B. Buck, et al.
Gepubliceerd in: (2008-06-01)

Study on Suitability of KOD DNA Polymerase for Enzymatic Production of Artificial Nucleic Acids Using Base/Sugar Modified Nucleoside Triphosphates
door: Satoshi Obika, et al.
Gepubliceerd in: (2010-11-01)

Simultaneous detection of FISH signals and bromo-deoxyuridine incorporation in fixed tissue cultured cells.
door: Moralli, D, et al.
Gepubliceerd in: (2009)

Simultaneous detection of FISH signals and bromo-deoxyuridine incorporation in fixed tissue cultured cells.
door: Daniela Moralli, et al.
Gepubliceerd in: (2009-01-01)

Improved synthesis of 5-hydroxymethyl-2'-deoxycytidine phosphoramidite using a 2'-deoxyuridine to 2'-deoxycytidine conversion without temporary protecting groups.
door: Hansen, A, et al.
Gepubliceerd in: (2011)

Alkyne-Functionalized Cyclooctyne on Si(001): Reactivity Studies and Surface Bonding from an Energy Decomposition Analysis Perspective
door: Fabian Pieck, et al.
Gepubliceerd in: (2021-11-01)

Synthesis, oligonucleotide incorporation and fluorescence properties in DNA of a bicyclic thymine analogue
door: Lawson, CP, et al.
Gepubliceerd in: (2018)

Enzymatic synthesis of chemically modified DNA for analytical and diagnostic applications
door: Ren, X
Gepubliceerd in: (2015)

Selective and Sensitive Fluorescence Aptamer Biosensors of Adenosine Triphosphate
door: Chao Meng, et al.
Gepubliceerd in: (2016-05-01)

Ethyl deoxyuridine in the management of herpes simplex keratitis
door: Malik SRK, et al.
Gepubliceerd in: (1979-01-01)

Synthesis, oligonucleotide incorporation and fluorescence properties in DNA of a bicyclic thymine analogue
door: Christopher P. Lawson, et al.
Gepubliceerd in: (2018-09-01)

Modified Nucleoside Triphosphates for in-vitro Selection Techniques
door: Adolfo Marcelo Iribarren, et al.
Gepubliceerd in: (2016-05-01)

Ex vivo and in vivo fluorescence detection and imaging of adenosine triphosphate
door: Binbin Chu, et al.
Gepubliceerd in: (2021-06-01)

5-(1-propargylamino)-2'-deoxyuridine (UP): a novel thymidine analogue for generating DNA triplexes with increased stability.
door: Bijapur, J, et al.
Gepubliceerd in: (1999)

Organometallic Nucleosides: Synthesis and Biological Evaluation of Substituted Dicobalt Hexacarbonyl Alkynyl Modified 2′-Deoxyuridines
door: Roman Dembinski, et al.
Gepubliceerd in: (2019-08-01)

5-Bromo-2’-deoxyuridine labeling: historical perspectives, factors influencing the detection, toxicity, and its implications in the neurogenesis
door: Joaquín Martí-Clúa
Gepubliceerd in: (2024-01-01)

A Simple Colorimetric and Fluorescent Sensor to Detect Organophosphate Pesticides Based on Adenosine Triphosphate-Modified Gold Nanoparticles
door: Xiaoxia Li, et al.
Gepubliceerd in: (2018-12-01)

Fast and efficient DNA crosslinking and multiple orthogonal labelling by copper-free click chemistry.
door: Shelbourne, M, et al.
Gepubliceerd in: (2012)