Simultaneous viral whole-genome sequencing and differential expression profiling in respiratory syncytial virus infection of infants
Targeted metagenomics using strand-specific libraries with target enrichment is a sensitive, generalized approach to pathogen sequencing and transcriptome profiling. Using this method, we recovered 13 (76%) complete human respiratory syncytial virus (RSV) genomes from 17 clinical respiratory samples...
| Main Authors: | , , , , , , , , , , , , , , |
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| Other Authors: | |
| Format: | Journal article |
| Language: | English |
| Published: |
Oxford University Press
2020
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| _version_ | 1826317661014851584 |
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| author | Lin, G-L Golubchik, T Drysdale, S O'Connor, D Jefferies, K Brown, A de Cesare, M Bonsall, D Ansari, MA Aerssens, J Bont, L Openshaw, P Martinón-Torres, F Bowden, R Pollard, AJ |
| author2 | RESCEU Investigators |
| author_facet | RESCEU Investigators Lin, G-L Golubchik, T Drysdale, S O'Connor, D Jefferies, K Brown, A de Cesare, M Bonsall, D Ansari, MA Aerssens, J Bont, L Openshaw, P Martinón-Torres, F Bowden, R Pollard, AJ |
| author_sort | Lin, G-L |
| collection | OXFORD |
| description | Targeted metagenomics using strand-specific libraries with target enrichment is a sensitive, generalized approach to pathogen sequencing and transcriptome profiling. Using this method, we recovered 13 (76%) complete human respiratory syncytial virus (RSV) genomes from 17 clinical respiratory samples, reconstructed the phylogeny of the infecting viruses, and detected differential gene expression between two RSV subgroups, specifically, a lower expression of the P gene and a higher expression of the M2 gene in RSV-A than in RSV-B. This methodology can help to relate viral genetics to clinical phenotype and facilitate ongoing population-level RSV surveillance and vaccine development. |
| first_indexed | 2024-03-07T05:02:56Z |
| format | Journal article |
| id | oxford-uuid:d8f0a045-819c-4027-b879-9e05f432604f |
| institution | University of Oxford |
| language | English |
| last_indexed | 2025-03-11T16:57:26Z |
| publishDate | 2020 |
| publisher | Oxford University Press |
| record_format | dspace |
| spelling | oxford-uuid:d8f0a045-819c-4027-b879-9e05f432604f2025-02-24T13:14:48ZSimultaneous viral whole-genome sequencing and differential expression profiling in respiratory syncytial virus infection of infantsJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:d8f0a045-819c-4027-b879-9e05f432604fEnglishSymplectic ElementsOxford University Press2020Lin, G-LGolubchik, TDrysdale, SO'Connor, DJefferies, KBrown, Ade Cesare, MBonsall, DAnsari, MAAerssens, JBont, LOpenshaw, PMartinón-Torres, FBowden, RPollard, AJRESCEU InvestigatorsTargeted metagenomics using strand-specific libraries with target enrichment is a sensitive, generalized approach to pathogen sequencing and transcriptome profiling. Using this method, we recovered 13 (76%) complete human respiratory syncytial virus (RSV) genomes from 17 clinical respiratory samples, reconstructed the phylogeny of the infecting viruses, and detected differential gene expression between two RSV subgroups, specifically, a lower expression of the P gene and a higher expression of the M2 gene in RSV-A than in RSV-B. This methodology can help to relate viral genetics to clinical phenotype and facilitate ongoing population-level RSV surveillance and vaccine development. |
| spellingShingle | Lin, G-L Golubchik, T Drysdale, S O'Connor, D Jefferies, K Brown, A de Cesare, M Bonsall, D Ansari, MA Aerssens, J Bont, L Openshaw, P Martinón-Torres, F Bowden, R Pollard, AJ Simultaneous viral whole-genome sequencing and differential expression profiling in respiratory syncytial virus infection of infants |
| title | Simultaneous viral whole-genome sequencing and differential expression profiling in respiratory syncytial virus infection of infants |
| title_full | Simultaneous viral whole-genome sequencing and differential expression profiling in respiratory syncytial virus infection of infants |
| title_fullStr | Simultaneous viral whole-genome sequencing and differential expression profiling in respiratory syncytial virus infection of infants |
| title_full_unstemmed | Simultaneous viral whole-genome sequencing and differential expression profiling in respiratory syncytial virus infection of infants |
| title_short | Simultaneous viral whole-genome sequencing and differential expression profiling in respiratory syncytial virus infection of infants |
| title_sort | simultaneous viral whole genome sequencing and differential expression profiling in respiratory syncytial virus infection of infants |
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