Subunit exchange of multimeric protein complexes. Real-time monitoring of subunit exchange between small heat shock proteins by using electrospray mass spectrometry

The subunit exchange of the small heat shock proteins (sHSPs) PsHSP18.1 from pea and TaHSP16.9 from wheat has been monitored in real-time using nanoelectrospray mass spectrometry. By preserving the noncovalent interactions between subunits in the mass spectrometer, we show that these proteins are do...

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Bibliographic Details
Main Authors: Sobott, F, Benesch, J, Vierling, E, Robinson, C
Format: Journal article
Language:English
Published: American Society for Biochemistry and Molecular Biology 2002