Polyfunctional cytomegalovirus-specific CD4+ and pp65 CD8+ T cells protect against high-level replication after liver transplantation.
To determine whether polyfunctional CD4+ T-cell responses coupled with CD8+ T-cell responses against human cytomegalovirus (HCMV) are key to the control of HCMV replication we prospectively analyzed 29 liver transplant recipients for CD4+ T-cell responses against soluble HCMV antigen, pp65 and IE1 p...
| Asıl Yazarlar: | , , , , , , , , |
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| Materyal Türü: | Journal article |
| Dil: | English |
| Baskı/Yayın Bilgisi: |
2008
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| _version_ | 1826300439891542016 |
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| author | Nebbia, G Mattes, F Smith, C Hainsworth, E Kopycinski, J Burroughs, A Griffiths, P Klenerman, P Emery, VC |
| author_facet | Nebbia, G Mattes, F Smith, C Hainsworth, E Kopycinski, J Burroughs, A Griffiths, P Klenerman, P Emery, VC |
| author_sort | Nebbia, G |
| collection | OXFORD |
| description | To determine whether polyfunctional CD4+ T-cell responses coupled with CD8+ T-cell responses against human cytomegalovirus (HCMV) are key to the control of HCMV replication we prospectively analyzed 29 liver transplant recipients for CD4+ T-cell responses against soluble HCMV antigen, pp65 and IE1 proteins, CD8+ T-cell responses against pp65 and IE1 proteins and a range of T helper (Th) 1 and Th2 cytokines. Eleven patients (38%) developed HCMV DNAemia at a median of 21 days post-liver transplantation (range 17-31 days). There was a significantly lower frequency and absolute number of total HCMV CD4+ T cells producing IFNgamma, IFNgamma+IL2 and IL2 and pp65-CD8+ T cells producing IFNgamma in patients with DNAemia. The quantities of Th1 and Th2 cytokines present during the first 20 days posttransplant were not predictive of DNAemia. Cut-off levels during the first 20 days posttransplant of 0.1% of lysate stimulated CD4+ T cells producing IL2, and pp65-CD8+ T cells producing IFNgamma above 0.4% had positive and negative predictive values for DNAemia of 54% and 100% and 50% and 92%, respectively. Measuring polyfunctional CD4+ T cells against HCMV early posttransplant may allow targeted intervention to minimize the occurrence and acute and long-term consequences of HCMV replication. |
| first_indexed | 2024-03-07T05:17:12Z |
| format | Journal article |
| id | oxford-uuid:dd9df2c0-2d9e-4e46-bde6-e99df0cabdd5 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T05:17:12Z |
| publishDate | 2008 |
| record_format | dspace |
| spelling | oxford-uuid:dd9df2c0-2d9e-4e46-bde6-e99df0cabdd52022-03-27T09:26:20ZPolyfunctional cytomegalovirus-specific CD4+ and pp65 CD8+ T cells protect against high-level replication after liver transplantation.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:dd9df2c0-2d9e-4e46-bde6-e99df0cabdd5EnglishSymplectic Elements at Oxford2008Nebbia, GMattes, FSmith, CHainsworth, EKopycinski, JBurroughs, AGriffiths, PKlenerman, PEmery, VCTo determine whether polyfunctional CD4+ T-cell responses coupled with CD8+ T-cell responses against human cytomegalovirus (HCMV) are key to the control of HCMV replication we prospectively analyzed 29 liver transplant recipients for CD4+ T-cell responses against soluble HCMV antigen, pp65 and IE1 proteins, CD8+ T-cell responses against pp65 and IE1 proteins and a range of T helper (Th) 1 and Th2 cytokines. Eleven patients (38%) developed HCMV DNAemia at a median of 21 days post-liver transplantation (range 17-31 days). There was a significantly lower frequency and absolute number of total HCMV CD4+ T cells producing IFNgamma, IFNgamma+IL2 and IL2 and pp65-CD8+ T cells producing IFNgamma in patients with DNAemia. The quantities of Th1 and Th2 cytokines present during the first 20 days posttransplant were not predictive of DNAemia. Cut-off levels during the first 20 days posttransplant of 0.1% of lysate stimulated CD4+ T cells producing IL2, and pp65-CD8+ T cells producing IFNgamma above 0.4% had positive and negative predictive values for DNAemia of 54% and 100% and 50% and 92%, respectively. Measuring polyfunctional CD4+ T cells against HCMV early posttransplant may allow targeted intervention to minimize the occurrence and acute and long-term consequences of HCMV replication. |
| spellingShingle | Nebbia, G Mattes, F Smith, C Hainsworth, E Kopycinski, J Burroughs, A Griffiths, P Klenerman, P Emery, VC Polyfunctional cytomegalovirus-specific CD4+ and pp65 CD8+ T cells protect against high-level replication after liver transplantation. |
| title | Polyfunctional cytomegalovirus-specific CD4+ and pp65 CD8+ T cells protect against high-level replication after liver transplantation. |
| title_full | Polyfunctional cytomegalovirus-specific CD4+ and pp65 CD8+ T cells protect against high-level replication after liver transplantation. |
| title_fullStr | Polyfunctional cytomegalovirus-specific CD4+ and pp65 CD8+ T cells protect against high-level replication after liver transplantation. |
| title_full_unstemmed | Polyfunctional cytomegalovirus-specific CD4+ and pp65 CD8+ T cells protect against high-level replication after liver transplantation. |
| title_short | Polyfunctional cytomegalovirus-specific CD4+ and pp65 CD8+ T cells protect against high-level replication after liver transplantation. |
| title_sort | polyfunctional cytomegalovirus specific cd4 and pp65 cd8 t cells protect against high level replication after liver transplantation |
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