Quantitating T cell cross-reactivity for unrelated peptide antigens.
Quantitating the frequency of T cell cross-reactivity to unrelated peptides is essential to understanding T cell responses in infectious and autoimmune diseases. Here we used 15 mouse or human CD8+ T cell clones (11 antiviral, 4 anti-self) in conjunction with a large library of defined synthetic pep...
| Hlavní autoři: | , , , , , , , , , , , , , , , , |
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| Médium: | Journal article |
| Jazyk: | English |
| Vydáno: |
2009
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| _version_ | 1826300662375251968 |
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| author | Ishizuka, J Grebe, K Shenderov, E Peters, B Chen, Q Peng, Y Wang, L Dong, T Pasquetto, V Oseroff, C Sidney, J Hickman, H Cerundolo, V Sette, A Bennink, JR McMichael, A Yewdell, J |
| author_facet | Ishizuka, J Grebe, K Shenderov, E Peters, B Chen, Q Peng, Y Wang, L Dong, T Pasquetto, V Oseroff, C Sidney, J Hickman, H Cerundolo, V Sette, A Bennink, JR McMichael, A Yewdell, J |
| author_sort | Ishizuka, J |
| collection | OXFORD |
| description | Quantitating the frequency of T cell cross-reactivity to unrelated peptides is essential to understanding T cell responses in infectious and autoimmune diseases. Here we used 15 mouse or human CD8+ T cell clones (11 antiviral, 4 anti-self) in conjunction with a large library of defined synthetic peptides to examine nearly 30,000 TCR-peptide MHC class I interactions for cross-reactions. We identified a single cross-reaction consisting of an anti-self TCR recognizing a poxvirus peptide at relatively low sensitivity. We failed to identify any cross-reactions between the synthetic peptides in the panel and polyclonal CD8+ T cells raised to viral or alloantigens. These findings provide the best estimate to date of the frequency of T cell cross-reactivity to unrelated peptides ( approximately 1/30,000), explaining why cross-reactions between unrelated pathogens are infrequently encountered and providing a critical parameter for understanding the scope of self-tolerance. |
| first_indexed | 2024-03-07T05:20:34Z |
| format | Journal article |
| id | oxford-uuid:debf8ecd-68cb-4203-9767-9a585d03841d |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T05:20:34Z |
| publishDate | 2009 |
| record_format | dspace |
| spelling | oxford-uuid:debf8ecd-68cb-4203-9767-9a585d03841d2022-03-27T09:34:29ZQuantitating T cell cross-reactivity for unrelated peptide antigens.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:debf8ecd-68cb-4203-9767-9a585d03841dEnglishSymplectic Elements at Oxford2009Ishizuka, JGrebe, KShenderov, EPeters, BChen, QPeng, YWang, LDong, TPasquetto, VOseroff, CSidney, JHickman, HCerundolo, VSette, ABennink, JRMcMichael, AYewdell, JQuantitating the frequency of T cell cross-reactivity to unrelated peptides is essential to understanding T cell responses in infectious and autoimmune diseases. Here we used 15 mouse or human CD8+ T cell clones (11 antiviral, 4 anti-self) in conjunction with a large library of defined synthetic peptides to examine nearly 30,000 TCR-peptide MHC class I interactions for cross-reactions. We identified a single cross-reaction consisting of an anti-self TCR recognizing a poxvirus peptide at relatively low sensitivity. We failed to identify any cross-reactions between the synthetic peptides in the panel and polyclonal CD8+ T cells raised to viral or alloantigens. These findings provide the best estimate to date of the frequency of T cell cross-reactivity to unrelated peptides ( approximately 1/30,000), explaining why cross-reactions between unrelated pathogens are infrequently encountered and providing a critical parameter for understanding the scope of self-tolerance. |
| spellingShingle | Ishizuka, J Grebe, K Shenderov, E Peters, B Chen, Q Peng, Y Wang, L Dong, T Pasquetto, V Oseroff, C Sidney, J Hickman, H Cerundolo, V Sette, A Bennink, JR McMichael, A Yewdell, J Quantitating T cell cross-reactivity for unrelated peptide antigens. |
| title | Quantitating T cell cross-reactivity for unrelated peptide antigens. |
| title_full | Quantitating T cell cross-reactivity for unrelated peptide antigens. |
| title_fullStr | Quantitating T cell cross-reactivity for unrelated peptide antigens. |
| title_full_unstemmed | Quantitating T cell cross-reactivity for unrelated peptide antigens. |
| title_short | Quantitating T cell cross-reactivity for unrelated peptide antigens. |
| title_sort | quantitating t cell cross reactivity for unrelated peptide antigens |
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