Three-dimensional STED microscopy of aberrating tissue using dual adaptive optics

When imaging through tissue, the optical inhomogeneities of the sample generate aberrations that can prevent effective Stimulated Emission Depletion (STED) imaging. This is particularly problematic for 3D-enhanced STED. We present here an adaptive optics implementation that incorporates two adaptive...

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Detalhes bibliográficos
Principais autores: Patton, B, Booth, M, Owald, D, Burke, D, Gould, T, Bewersdorf, J
Formato: Journal article
Publicado em: Optical Society of America 2016