An optimized protocol for expression and purification of murine perforin in insect cells

Perforin (PFN) is one of the most important protein effectors of the immune system. It is produced by cytotoxic T lymphocytes and natural killer cells and helps with the clearance of virus-infected and tumor cells. PFN is a pore-forming protein that readily binds to the lipid membranes of target cel...

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Bibliographic Details
Main Authors: Naneh, O, Zavec, A, Pahovnik, D, Zagar, E, Gilbert, R, Krizaj, I, Anderluh, G
Format: Journal article
Published: Elsevier 2015