Probing distance and electrical potential within a protein pore with tethered DNA.
DNA molecules tethered inside a protein pore can be used as a tool to probe distance and electrical potential. The approach and its limitations were tested with alpha-hemolysin, a pore of known structure. A single oligonucleotide was attached to an engineered cysteine to allow the binding of complem...
| Main Authors: | , |
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| Format: | Journal article |
| Language: | English |
| Published: |
2002
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