Multiphoton-FLIM quantification of the EGFP-mRFP1 FRET pair for localization of membrane receptor-kinase interactions.

Multiphoton-FLIM quantification of the EGFP-mRFP1 FRET pair for localization of membrane receptor-kinase interactions.

We present an improved monomeric form of the red fluorescent protein, mRFP1, as the acceptor in biological fluorescence resonance energy transfer (FRET) experiments using the enhanced green fluorescent protein as donor. We find particular advantage in using this fluorophore pair for quantitative mea...

Πλήρης περιγραφή

Λεπτομέρειες βιβλιογραφικής εγγραφής
Κύριοι συγγραφείς:	Peter, M, Ameer-Beg, S, Hughes, M, Keppler, MD, Prag, S, Marsh, M, Vojnovic, B, Ng, T
Μορφή:	Journal article
Γλώσσα:	English
Έκδοση:	2005

Παρόμοια τεκμήρια

Dynamic imaging of protein-protein interactions by MP-FLIM
ανά: Ameer-Beg, S, κ.ά.
Έκδοση: (2005)

Global and pixel kinetic data analysis for FRET detection by multi-photon time-domain FLIM
ανά: Barber, P, κ.ά.
Έκδοση: (2005)

Practical and reliable FRET/FLIM pair of fluorescent proteins
ανά: Shemiakina Irina I, κ.ά.
Έκδοση: (2009-03-01)

Noninvasive Monitoring of mRFP1- and mCherry-Labeled Oncolytic Adenoviruses in an Orthotopic Breast Cancer Model by Spectral Imaging
ανά: Anton V. Borovjagin, κ.ά.
Έκδοση: (2010-03-01)

Biolistic Bombardment for Co-expression of Proteins Fused to YFP and mRFP in Leaf Epidermal Cells of Phaseolus vulgaris ‘Red Mexican’
ανά: Malgorzata Lichocka
Έκδοση: (2014-01-01)

In vivo melanin 3D quantification and z-epidermal distribution by multiphoton FLIM, phasor and Pseudo-FLIM analyses
ανά: Ana-Maria Pena, κ.ά.
Έκδοση: (2022-01-01)

Effect of phosphorylation on EGFR dimer stability probed by single-molecule dynamics and FRET/FLIM
ανά: Coban, O, κ.ά.
Έκδοση: (2015)

The Humanised NPY-mRFP RBL Reporter Cell Line Is a Fast and Inexpensive Tool for Detection of Allergen-Specific IgE in Human Sera
ανά: Prema S. Prakash, κ.ά.
Έκδοση: (2022-08-01)

Implementation and application of FRET–FLIM technology
ανά: Shiqi Wang, κ.ά.
Έκδοση: (2019-09-01)

A high-content screening platform utilizing polarization anisotropy and FLIM microscopy
ανά: Matthews, DR, κ.ά.
Έκδοση: (2008)

Single cell FRET analysis for the identification of optimal FRET-pairs in Bacillus subtilis using a prototype MEM-FLIM system.
ανά: Ruud G J Detert Oude Weme, κ.ά.
Έκδοση: (2015-01-01)

Multiphoton time-domain fluorescence lifetime imaging microscopy: practical application to protein-protein interactions using global analysis
ανά: Barber, P, κ.ά.
Έκδοση: (2009)

Subcellular Localization Experiments and FRET-FLIM Measurements in Plants
ανά: Malgorzata Lichocka, κ.ά.
Έκδοση: (2014-01-01)

HER2-HER3 dimer quantification by FLIM-FRET predicts breast cancer metastatic relapse independently of HER2 IHC status
ανά: Vojnovic, B, κ.ά.
Έκδοση: (2016)

Towards high-throughput flim for protein-protein interaction screening of live cells and tissue microarrays
ανά: Barber, P, κ.ά.
Έκδοση: (2008)

Imaging protein-protein interactions in cell motility using fluorescence resonance energy transfer (FRET).
ανά: Parsons, M, κ.ά.
Έκδοση: (2004)

Methodological considerations for global analysis of cellular FLIM/FRET measurements
ανά: Rahim, Nur Aida Adbul, κ.ά.
Έκδοση: (2012)

Mapping femtosecond pulse front distortion and group velocity dispersion in multiphoton microscopy
ανά: Tullis, I, κ.ά.
Έκδοση: (2006)

Mapping femtosecond pulse front distortion and group velocity dispersion in multiphoton microscopy
ανά: Tullis, I, κ.ά.
Έκδοση: (2006)

Assessment of EGFR/HER2 dimerization by FRET-FLIM utilizing Alexa-conjugated secondary antibodies in relation to targeted therapies in cancers.
ανά: Waterhouse, B, κ.ά.
Έκδοση: (2011)

Quantitative comparison of different fluorescent protein couples for fast FRET-FLIM acquisition.
ανά: Padilla-Parra, S, κ.ά.
Έκδοση: (2009)

Advanced Fluorescence Microscopy Techniques—FRAP, FLIP, FLAP, FRET and FLIM
ανά: Gregor P. C. Drummen, κ.ά.
Έκδοση: (2012-04-01)

An improved Ras sensor for highly sensitive and quantitative FRET-FLIM imaging.
ανά: Ana F Oliveira, κ.ά.
Έκδοση: (2013-01-01)

827Spatio-Temporal Quantification of FRET in living cells by fast time-domain FLIM: a comparative study of non-fitting methods
ανά: Leray, A, κ.ά.
Έκδοση: (2013)

Accepting from the best donor; analysis of long-lifetime donor fluorescent protein pairings to optimise dynamic FLIM-based FRET experiments.
ανά: Kirsty J Martin, κ.ά.
Έκδοση: (2018-01-01)

Epigenetic biomarker screening by FLIM-FRET for combination therapy in ER+ breast cancer
ανά: Wenjie Liu, κ.ά.
Έκδοση: (2019-01-01)

Imaging of Metabolic Status in 3D Cultures with an Improved AMPK FRET Biosensor for FLIM
ανά: George Chennell, κ.ά.
Έκδοση: (2016-08-01)

Correction: 827Spatio-Temporal Quantification of FRET in Living Cells by Fast Time-Domain FLIM: A Comparative Study of Non-Fitting Methods.
ανά: Leray, A, κ.ά.
Έκδοση: (2013)

RfpA, RfpB, and RfpC are the Master Control Elements of Far-Red Light Photoacclimation (FaRLiP)
ανά: Chi Zhao, κ.ά.
Έκδοση: (2015-11-01)

Spatio-Temporal Quantification of FRET in living cells by fast time-domain FLIM: a comparative study of non-fitting methods [corrected].
ανά: Aymeric Leray, κ.ά.
Έκδοση: (2013-01-01)

Time-lapse FRET microscopy using fluorescence anisotropy.
ανά: Matthews, DR, κ.ά.
Έκδοση: (2010)

Temporal Data Set Reduction Based on D-Optimality for Quantitative FLIM-FRET Imaging.
ανά: Travis Omer, κ.ά.
Έκδοση: (2015-01-01)

Visualizing multimerization of plasticity-related gene 5 at the plasma membrane using FLIM-FRET
ανά: Franziska Köper, κ.ά.
Έκδοση: (2024-09-01)

FLIM‐FRET‐based analysis of S100A11/annexin interactions in living cells
ανά: Christian Melle, κ.ά.
Έκδοση: (2024-04-01)

Using FLIM-FRET to measure conformational changes of transglutaminase type 2 in live cells.
ανά: Nicholas S Caron, κ.ά.
Έκδοση: (2012-01-01)

ShadowY: a dark yellow fluorescent protein for FLIM-based FRET measurement
ανά: Hideji Murakoshi, κ.ά.
Έκδοση: (2017-07-01)

Measuring Small-molecule Inhibition of Protein Interactions in Live Cells Using FLIM-FRET
ανά: James Pemberton, κ.ά.
Έκδοση: (2019-10-01)

Corrigendum: RfpA, RfpB, and RfpC are the Master Control Elements of Far-Red Light Photoacclimation (FaRLiP)
ανά: Chi Zhao, κ.ά.
Έκδοση: (2019-03-01)

Quantitative FRET analysis by fast acquisition time domain FLIM at high spatial resolution in living cells.
ανά: Padilla-Parra, S, κ.ά.
Έκδοση: (2008)

Correction: 827Spatio-Temporal Quantification of FRET in Living Cells by Fast Time-Domain FLIM: A Comparative Study of Non-Fitting Methods.
ανά: Aymeric Leray, κ.ά.
Έκδοση: (2013-01-01)