Optical sectioning in fluorescence microscopy.
We review the origins of optical sectioning in fluorescence microscopy in terms of the structure of the illumination used to generate the fluorescence within the specimen. We note that the conventional microscope using essentially uniform illumination does not exhibit optical sectioning whereas the...
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| Formaat: | Journal article |
| Taal: | English |
| Gepubliceerd in: |
2011
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