CpG-free plasmids confer reduced inflammation and sustained pulmonary gene expression.

CpG-free plasmids confer reduced inflammation and sustained pulmonary gene expression.

Pulmonary delivery of plasmid DNA (pDNA)/cationic liposome complexes is associated with an acute unmethylated CG dinucleotide (CpG)-mediated inflammatory response and brief duration of transgene expression. We demonstrate that retention of even a single CpG in pDNA is sufficient to elicit an inflamm...

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Hlavní autoři: Hyde, S, Pringle, I, Abdullah, S, Lawton, A, Davies, L, Varathalingam, A, Nunez-Alonso, G, Green, A, Bazzani, R, Sumner-Jones, S, Chan, M, Li, H, Yew, N, Cheng, S, Boyd, A, Davies, J, Griesenbach, U, Porteous, D, Sheppard, D, Munkonge, F, Alton, E, Gill, D
Médium: Journal article
Jazyk:English
Vydáno: 2008
Popis
Shrnutí:Pulmonary delivery of plasmid DNA (pDNA)/cationic liposome complexes is associated with an acute unmethylated CG dinucleotide (CpG)-mediated inflammatory response and brief duration of transgene expression. We demonstrate that retention of even a single CpG in pDNA is sufficient to elicit an inflammatory response, whereas CpG-free pDNA vectors do not. Using a CpG-free pDNA expression vector, we achieved sustained (>or=56 d) in vivo transgene expression in the absence of lung inflammation.

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