A quantitative assay of peptide-dependent class I assembly.
We have developed a quantitative assay for the measurement of class I assembly induced by peptide. We have applied this assay to H-2Db, Kb and HLA-A2.1 with a panel of 49 overlapping peptides derived from HIV-1 gag protein. We find that the effects of peptide on assembly form a continuous distributi...
| Main Authors: | , , , |
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| Format: | Journal article |
| Language: | English |
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1991
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| _version_ | 1826301580080578560 |
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| author | Elvin, J Cerundolo, V Elliott, T Townsend, A |
| author_facet | Elvin, J Cerundolo, V Elliott, T Townsend, A |
| author_sort | Elvin, J |
| collection | OXFORD |
| description | We have developed a quantitative assay for the measurement of class I assembly induced by peptide. We have applied this assay to H-2Db, Kb and HLA-A2.1 with a panel of 49 overlapping peptides derived from HIV-1 gag protein. We find that the effects of peptide on assembly form a continuous distribution. By defining positives as those that increase the concentration of folded heavy chains more than three standard deviations from the control we show that 7/48 bind A2.1, 11/49 bind Db and 7/47 bind Kb. The assembly assay contrasts with solid-phase assays in being more discriminating (fewer peptides binding any given class I molecule), and showing less overlap in the patterns of peptides bound by the three class I molecules. |
| first_indexed | 2024-03-07T05:34:33Z |
| format | Journal article |
| id | oxford-uuid:e36fde42-4bba-4609-a785-2a8db967cc66 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T05:34:33Z |
| publishDate | 1991 |
| record_format | dspace |
| spelling | oxford-uuid:e36fde42-4bba-4609-a785-2a8db967cc662022-03-27T10:09:06ZA quantitative assay of peptide-dependent class I assembly.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:e36fde42-4bba-4609-a785-2a8db967cc66EnglishSymplectic Elements at Oxford1991Elvin, JCerundolo, VElliott, TTownsend, AWe have developed a quantitative assay for the measurement of class I assembly induced by peptide. We have applied this assay to H-2Db, Kb and HLA-A2.1 with a panel of 49 overlapping peptides derived from HIV-1 gag protein. We find that the effects of peptide on assembly form a continuous distribution. By defining positives as those that increase the concentration of folded heavy chains more than three standard deviations from the control we show that 7/48 bind A2.1, 11/49 bind Db and 7/47 bind Kb. The assembly assay contrasts with solid-phase assays in being more discriminating (fewer peptides binding any given class I molecule), and showing less overlap in the patterns of peptides bound by the three class I molecules. |
| spellingShingle | Elvin, J Cerundolo, V Elliott, T Townsend, A A quantitative assay of peptide-dependent class I assembly. |
| title | A quantitative assay of peptide-dependent class I assembly. |
| title_full | A quantitative assay of peptide-dependent class I assembly. |
| title_fullStr | A quantitative assay of peptide-dependent class I assembly. |
| title_full_unstemmed | A quantitative assay of peptide-dependent class I assembly. |
| title_short | A quantitative assay of peptide-dependent class I assembly. |
| title_sort | quantitative assay of peptide dependent class i assembly |
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