Detection of HIV type 1 gag-specific CD4(+) T cell responses in acutely infected infants.
Multiple HIV-1-specific cytokine and proliferative responses by CD4(+) T cells have not been studied in acutely infected infants. Using an intracellular cytokine staining assay, 34 untreated clade C HIV-1-infected infants (2-102 days old) were assessed for IFN-gamma, 28/34 for IL-2, and 26/34 for TN...
Main Authors: | , , , , , , , , , , , , , , , , , , |
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Format: | Journal article |
Language: | English |
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2008
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author | Ramduth, D Thobakgale, C Mkhwanazi, N De Pierres, C Reddy, S van der Stok, M Mncube, Z Mphatswe, W Blanckenberg, N Cengimbo, A Prendergast, A Tudor-Williams, G Dong, K Jeena, P Coovadia, H Day, C Kiepiela, P Goulder, P Walker, B |
author_facet | Ramduth, D Thobakgale, C Mkhwanazi, N De Pierres, C Reddy, S van der Stok, M Mncube, Z Mphatswe, W Blanckenberg, N Cengimbo, A Prendergast, A Tudor-Williams, G Dong, K Jeena, P Coovadia, H Day, C Kiepiela, P Goulder, P Walker, B |
author_sort | Ramduth, D |
collection | OXFORD |
description | Multiple HIV-1-specific cytokine and proliferative responses by CD4(+) T cells have not been studied in acutely infected infants. Using an intracellular cytokine staining assay, 34 untreated clade C HIV-1-infected infants (2-102 days old) were assessed for IFN-gamma, 28/34 for IL-2, and 26/34 for TNF-alpha responses to all HIV-1 proteins. Responses were detected in 29%, 36%, and 15% of infants, respectively. Twelve of the original 34 infants were then studied longitudinally for 14 months to determine the effect of viral load on IFN-gamma Gag-specific responses: seven infants were treated for 1 year, stopped treatment, and resumed when CD4% was < 20 and five infants were treated only when the CD4% was <20. Following treatment cessation, there was an immediate increase in viral load followed by an increase in the magnitude of CD4(+) Gag-specific responses. Despite this, the majority of infants (54%) had to restart treatment by 24 months of age, indicating that the immune responses were antigen driven but not associated with protection. Among untreated infants HIV-specific CD4(+) responses were detected sporadically indicating a dysfunctional immune response in the face of constant exposure to high levels of viremia. |
first_indexed | 2024-03-07T05:38:56Z |
format | Journal article |
id | oxford-uuid:e4e8d883-e512-4d5d-901c-c6ea4a28574f |
institution | University of Oxford |
language | English |
last_indexed | 2024-03-07T05:38:56Z |
publishDate | 2008 |
record_format | dspace |
spelling | oxford-uuid:e4e8d883-e512-4d5d-901c-c6ea4a28574f2022-03-27T10:20:00ZDetection of HIV type 1 gag-specific CD4(+) T cell responses in acutely infected infants.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:e4e8d883-e512-4d5d-901c-c6ea4a28574fEnglishSymplectic Elements at Oxford2008Ramduth, DThobakgale, CMkhwanazi, NDe Pierres, CReddy, Svan der Stok, MMncube, ZMphatswe, WBlanckenberg, NCengimbo, APrendergast, ATudor-Williams, GDong, KJeena, PCoovadia, HDay, CKiepiela, PGoulder, PWalker, BMultiple HIV-1-specific cytokine and proliferative responses by CD4(+) T cells have not been studied in acutely infected infants. Using an intracellular cytokine staining assay, 34 untreated clade C HIV-1-infected infants (2-102 days old) were assessed for IFN-gamma, 28/34 for IL-2, and 26/34 for TNF-alpha responses to all HIV-1 proteins. Responses were detected in 29%, 36%, and 15% of infants, respectively. Twelve of the original 34 infants were then studied longitudinally for 14 months to determine the effect of viral load on IFN-gamma Gag-specific responses: seven infants were treated for 1 year, stopped treatment, and resumed when CD4% was < 20 and five infants were treated only when the CD4% was <20. Following treatment cessation, there was an immediate increase in viral load followed by an increase in the magnitude of CD4(+) Gag-specific responses. Despite this, the majority of infants (54%) had to restart treatment by 24 months of age, indicating that the immune responses were antigen driven but not associated with protection. Among untreated infants HIV-specific CD4(+) responses were detected sporadically indicating a dysfunctional immune response in the face of constant exposure to high levels of viremia. |
spellingShingle | Ramduth, D Thobakgale, C Mkhwanazi, N De Pierres, C Reddy, S van der Stok, M Mncube, Z Mphatswe, W Blanckenberg, N Cengimbo, A Prendergast, A Tudor-Williams, G Dong, K Jeena, P Coovadia, H Day, C Kiepiela, P Goulder, P Walker, B Detection of HIV type 1 gag-specific CD4(+) T cell responses in acutely infected infants. |
title | Detection of HIV type 1 gag-specific CD4(+) T cell responses in acutely infected infants. |
title_full | Detection of HIV type 1 gag-specific CD4(+) T cell responses in acutely infected infants. |
title_fullStr | Detection of HIV type 1 gag-specific CD4(+) T cell responses in acutely infected infants. |
title_full_unstemmed | Detection of HIV type 1 gag-specific CD4(+) T cell responses in acutely infected infants. |
title_short | Detection of HIV type 1 gag-specific CD4(+) T cell responses in acutely infected infants. |
title_sort | detection of hiv type 1 gag specific cd4 t cell responses in acutely infected infants |
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