Detection of HIV type 1 gag-specific CD4(+) T cell responses in acutely infected infants.

Multiple HIV-1-specific cytokine and proliferative responses by CD4(+) T cells have not been studied in acutely infected infants. Using an intracellular cytokine staining assay, 34 untreated clade C HIV-1-infected infants (2-102 days old) were assessed for IFN-gamma, 28/34 for IL-2, and 26/34 for TN...

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Main Authors: Ramduth, D, Thobakgale, C, Mkhwanazi, N, De Pierres, C, Reddy, S, van der Stok, M, Mncube, Z, Mphatswe, W, Blanckenberg, N, Cengimbo, A, Prendergast, A, Tudor-Williams, G, Dong, K, Jeena, P, Coovadia, H, Day, C, Kiepiela, P, Goulder, P, Walker, B
Format: Journal article
Language:English
Published: 2008
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author Ramduth, D
Thobakgale, C
Mkhwanazi, N
De Pierres, C
Reddy, S
van der Stok, M
Mncube, Z
Mphatswe, W
Blanckenberg, N
Cengimbo, A
Prendergast, A
Tudor-Williams, G
Dong, K
Jeena, P
Coovadia, H
Day, C
Kiepiela, P
Goulder, P
Walker, B
author_facet Ramduth, D
Thobakgale, C
Mkhwanazi, N
De Pierres, C
Reddy, S
van der Stok, M
Mncube, Z
Mphatswe, W
Blanckenberg, N
Cengimbo, A
Prendergast, A
Tudor-Williams, G
Dong, K
Jeena, P
Coovadia, H
Day, C
Kiepiela, P
Goulder, P
Walker, B
author_sort Ramduth, D
collection OXFORD
description Multiple HIV-1-specific cytokine and proliferative responses by CD4(+) T cells have not been studied in acutely infected infants. Using an intracellular cytokine staining assay, 34 untreated clade C HIV-1-infected infants (2-102 days old) were assessed for IFN-gamma, 28/34 for IL-2, and 26/34 for TNF-alpha responses to all HIV-1 proteins. Responses were detected in 29%, 36%, and 15% of infants, respectively. Twelve of the original 34 infants were then studied longitudinally for 14 months to determine the effect of viral load on IFN-gamma Gag-specific responses: seven infants were treated for 1 year, stopped treatment, and resumed when CD4% was < 20 and five infants were treated only when the CD4% was <20. Following treatment cessation, there was an immediate increase in viral load followed by an increase in the magnitude of CD4(+) Gag-specific responses. Despite this, the majority of infants (54%) had to restart treatment by 24 months of age, indicating that the immune responses were antigen driven but not associated with protection. Among untreated infants HIV-specific CD4(+) responses were detected sporadically indicating a dysfunctional immune response in the face of constant exposure to high levels of viremia.
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spelling oxford-uuid:e4e8d883-e512-4d5d-901c-c6ea4a28574f2022-03-27T10:20:00ZDetection of HIV type 1 gag-specific CD4(+) T cell responses in acutely infected infants.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:e4e8d883-e512-4d5d-901c-c6ea4a28574fEnglishSymplectic Elements at Oxford2008Ramduth, DThobakgale, CMkhwanazi, NDe Pierres, CReddy, Svan der Stok, MMncube, ZMphatswe, WBlanckenberg, NCengimbo, APrendergast, ATudor-Williams, GDong, KJeena, PCoovadia, HDay, CKiepiela, PGoulder, PWalker, BMultiple HIV-1-specific cytokine and proliferative responses by CD4(+) T cells have not been studied in acutely infected infants. Using an intracellular cytokine staining assay, 34 untreated clade C HIV-1-infected infants (2-102 days old) were assessed for IFN-gamma, 28/34 for IL-2, and 26/34 for TNF-alpha responses to all HIV-1 proteins. Responses were detected in 29%, 36%, and 15% of infants, respectively. Twelve of the original 34 infants were then studied longitudinally for 14 months to determine the effect of viral load on IFN-gamma Gag-specific responses: seven infants were treated for 1 year, stopped treatment, and resumed when CD4% was < 20 and five infants were treated only when the CD4% was <20. Following treatment cessation, there was an immediate increase in viral load followed by an increase in the magnitude of CD4(+) Gag-specific responses. Despite this, the majority of infants (54%) had to restart treatment by 24 months of age, indicating that the immune responses were antigen driven but not associated with protection. Among untreated infants HIV-specific CD4(+) responses were detected sporadically indicating a dysfunctional immune response in the face of constant exposure to high levels of viremia.
spellingShingle Ramduth, D
Thobakgale, C
Mkhwanazi, N
De Pierres, C
Reddy, S
van der Stok, M
Mncube, Z
Mphatswe, W
Blanckenberg, N
Cengimbo, A
Prendergast, A
Tudor-Williams, G
Dong, K
Jeena, P
Coovadia, H
Day, C
Kiepiela, P
Goulder, P
Walker, B
Detection of HIV type 1 gag-specific CD4(+) T cell responses in acutely infected infants.
title Detection of HIV type 1 gag-specific CD4(+) T cell responses in acutely infected infants.
title_full Detection of HIV type 1 gag-specific CD4(+) T cell responses in acutely infected infants.
title_fullStr Detection of HIV type 1 gag-specific CD4(+) T cell responses in acutely infected infants.
title_full_unstemmed Detection of HIV type 1 gag-specific CD4(+) T cell responses in acutely infected infants.
title_short Detection of HIV type 1 gag-specific CD4(+) T cell responses in acutely infected infants.
title_sort detection of hiv type 1 gag specific cd4 t cell responses in acutely infected infants
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