A fluorescence-based assay for 2-oxoglutarate-dependent oxygenases.
A widely used generic assay for 2-oxoglutarate-dependent oxygenases relies upon monitoring the release of 14CO2 from labeled [1-14C]-2-oxoglutarate. We report an alternative assay in which depletion of 2-oxoglutarate is monitored by its postincubation derivatization with o-phenylenediamine to form a...
| Main Authors: | , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
2005
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