Refolding of recombinant Pneumocystis carinii dihydrofolate reductase and characterization of the enzyme.
The isolation of dihydrofolate reductase (DHFR) cDNA sequences from the messenger RNA of Pneumocystis carinii using the polymerase chain reaction is described. The 206-amino acid P. carinii DHFR was expressed to high levels in Escherichia coli inclusion bodies using the T7 promoter expression system...
| Main Authors: | , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
1993
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