Medium-throughput production of recombinant human proteins: Protein production in E. Coli
In Chapter 4 we described the SGC process for generating multiple constructs of truncated versions of each protein using LIC. In this chapter we provide a step-by-step procedure of our E. coli system for test expressing intracellular (soluble) proteins in a 96-well format that enables us to identify...
| Главные авторы: | , , , , |
|---|---|
| Формат: | Journal article |
| Язык: | English |
| Опубликовано: |
2014
|