Medium-throughput production of recombinant human proteins: Protein production in E. Coli
In Chapter 4 we described the SGC process for generating multiple constructs of truncated versions of each protein using LIC. In this chapter we provide a step-by-step procedure of our E. coli system for test expressing intracellular (soluble) proteins in a 96-well format that enables us to identify...
主要な著者: | Burgess-Brown, N, Mahajan, P, Strain-Damerell, C, Gileadi, O, Gräslund, S |
---|---|
フォーマット: | Journal article |
言語: | English |
出版事項: |
2014
|
類似資料
-
Medium-throughput production of recombinant human proteins: protein production in E. coli.
著者:: Burgess-Brown, N, 等
出版事項: (2014) -
Medium-throughput production of recombinant human proteins: Protein production in insect cells
著者:: Mahajan, P, 等
出版事項: (2014) -
Medium-throughput production of recombinant human proteins: protein production in insect cells.
著者:: Mahajan, P, 等
出版事項: (2014) -
Medium-throughput production of recombinant human proteins: ligation-independent cloning.
著者:: Strain-Damerell, C, 等
出版事項: (2014) -
Medium-throughput production of recombinant human proteins: Ligation-independent cloning
著者:: Strain-Damerell, C, 等
出版事項: (2014)