Medium-throughput production of recombinant human proteins: Protein production in E. Coli
In Chapter 4 we described the SGC process for generating multiple constructs of truncated versions of each protein using LIC. In this chapter we provide a step-by-step procedure of our E. coli system for test expressing intracellular (soluble) proteins in a 96-well format that enables us to identify...
المؤلفون الرئيسيون: | Burgess-Brown, N, Mahajan, P, Strain-Damerell, C, Gileadi, O, Gräslund, S |
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التنسيق: | Journal article |
اللغة: | English |
منشور في: |
2014
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مواد مشابهة
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Medium-throughput production of recombinant human proteins: protein production in E. coli.
حسب: Burgess-Brown, N, وآخرون
منشور في: (2014) -
Medium-throughput production of recombinant human proteins: Protein production in insect cells
حسب: Mahajan, P, وآخرون
منشور في: (2014) -
Medium-throughput production of recombinant human proteins: protein production in insect cells.
حسب: Mahajan, P, وآخرون
منشور في: (2014) -
Medium-throughput production of recombinant human proteins: ligation-independent cloning.
حسب: Strain-Damerell, C, وآخرون
منشور في: (2014) -
Medium-throughput production of recombinant human proteins: Ligation-independent cloning
حسب: Strain-Damerell, C, وآخرون
منشور في: (2014)