Medium-throughput production of recombinant human proteins: Protein production in E. Coli

Medium-throughput production of recombinant human proteins: Protein production in E. Coli

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In Chapter 4 we described the SGC process for generating multiple constructs of truncated versions of each protein using LIC. In this chapter we provide a step-by-step procedure of our E. coli system for test expressing intracellular (soluble) proteins in a 96-well format that enables us to identify...

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Bibliografiske detaljer
Main Authors:	Burgess-Brown, N, Mahajan, P, Strain-Damerell, C, Gileadi, O, Gräslund, S
Format:	Journal article
Sprog:	English
Udgivet:	2014

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