Medium-throughput production of recombinant human proteins: Protein production in E. Coli
In Chapter 4 we described the SGC process for generating multiple constructs of truncated versions of each protein using LIC. In this chapter we provide a step-by-step procedure of our E. coli system for test expressing intracellular (soluble) proteins in a 96-well format that enables us to identify...
| Autori principali: | , , , , |
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| Natura: | Journal article |
| Lingua: | English |
| Pubblicazione: |
2014
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Medium-throughput production of recombinant human proteins: protein production in E. coli.
Pubblicazione 2014
Journal article