Medium-throughput production of recombinant human proteins: Protein production in E. Coli

In Chapter 4 we described the SGC process for generating multiple constructs of truncated versions of each protein using LIC. In this chapter we provide a step-by-step procedure of our E. coli system for test expressing intracellular (soluble) proteins in a 96-well format that enables us to identify...

Disgrifiad llawn

Manylion Llyfryddiaeth
Prif Awduron: Burgess-Brown, N, Mahajan, P, Strain-Damerell, C, Gileadi, O, Gräslund, S
Fformat: Journal article
Iaith:English
Cyhoeddwyd: 2014