Medium-throughput production of recombinant human proteins: Protein production in E. Coli

In Chapter 4 we described the SGC process for generating multiple constructs of truncated versions of each protein using LIC. In this chapter we provide a step-by-step procedure of our E. coli system for test expressing intracellular (soluble) proteins in a 96-well format that enables us to identify...

Πλήρης περιγραφή

Λεπτομέρειες βιβλιογραφικής εγγραφής
Κύριοι συγγραφείς: Burgess-Brown, N, Mahajan, P, Strain-Damerell, C, Gileadi, O, Gräslund, S
Μορφή: Journal article
Γλώσσα:English
Έκδοση: 2014