A comparative study of digoxigenin, 2,4-dinitrophenyl, and alkaline phosphatase as deoxyoligonucleotide labels in non-radioisotopic in situ hybridisation.

AIM: To determine the optimum form of labelling and the most efficient reporter molecule for non-radioisotopic in situ hybridisation (ISH). METHODS: Nine deoxyoligonucleotides complementary to histone mRNA were synthesised and labelled either enzymatically or during solid-phase synthesis with the re...

وصف كامل

التفاصيل البيبلوغرافية
المؤلفون الرئيسيون: Harper, S, Bailey, E, McKeen, C, Stewart, A, Pringle, J, Feehally, J, Brown, T
التنسيق: Journal article
اللغة:English
منشور في: 1997