Gas-phase unfolding and disassembly reveals stability differences in ligand-bound multiprotein complexes.
Mass spectrometry (MS) is widely used to assess the binding of small molecules to proteins and their complexes. In many cases, subtle differences in the stability afforded by binding of ligands to protein assemblies cannot be detected by MS. Here we show that monitoring the unfolding of protein subu...
| Main Authors: | , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
2009
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