Chromosome-free bacterial cells are safe and programmable platforms for synthetic biology
A new type of chromosome-free cells called SimCells (simple cells) has been generated from Escherichia coli, Pseudomonas putida and Ralstonia eutropha. The removal of the native chromosomes of these bacteria was achieved by double-stranded breaks made by heterologous I-CeuI endonuclease, and the deg...
| Main Authors: | , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
National Academy of Sciences
2020
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