DNA triplex formation with 5-dimethylaminopropargyl deoxyuridine.
We have prepared triplex-forming oligonucleotides containing the nucleotide analogue 5-dimethylaminopropargyl deoxyuridine (DMAPdU) in place of thymidine and examined their ability to form intermolecular triple helices by thermal melting and DNase I footprinting studies. The results were compared wi...
| Asıl Yazarlar: | , , , , |
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| Materyal Türü: | Journal article |
| Dil: | English |
| Baskı/Yayın Bilgisi: |
2009
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| _version_ | 1826303470513160192 |
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| author | Rusling, D Peng, G Srinivasan, N Fox, K Brown, T |
| author_facet | Rusling, D Peng, G Srinivasan, N Fox, K Brown, T |
| author_sort | Rusling, D |
| collection | OXFORD |
| description | We have prepared triplex-forming oligonucleotides containing the nucleotide analogue 5-dimethylaminopropargyl deoxyuridine (DMAPdU) in place of thymidine and examined their ability to form intermolecular triple helices by thermal melting and DNase I footprinting studies. The results were compared with those for oligonucleotides containing 5-aminopropargyl-dU (APdU), 5-guanidinopropargyl-dU (GPdU) and 5-propynyl dU (PdU). We find that DMAPdU enhances triplex stability relative to T, though slightly less than the other analogues that bear positive charges (T << PdU < DMAPdU < APdU < GPdU). For oligonucleotides that contain multiple substitutions with DMAPdU dispersed residues are more effective than clustered combinations. DMAPdU will be especially useful as a nucleotide analogue as, unlike APdU and GPdU, the base does not require protection during oligonucleotide synthesis and it can therefore be used with other derivatives that require mild deprotection conditions. |
| first_indexed | 2024-03-07T06:03:11Z |
| format | Journal article |
| id | oxford-uuid:ecebf8f4-ad8b-4209-8df3-feb4020edf1c |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T06:03:11Z |
| publishDate | 2009 |
| record_format | dspace |
| spelling | oxford-uuid:ecebf8f4-ad8b-4209-8df3-feb4020edf1c2022-03-27T11:21:04ZDNA triplex formation with 5-dimethylaminopropargyl deoxyuridine.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:ecebf8f4-ad8b-4209-8df3-feb4020edf1cEnglishSymplectic Elements at Oxford2009Rusling, DPeng, GSrinivasan, NFox, KBrown, TWe have prepared triplex-forming oligonucleotides containing the nucleotide analogue 5-dimethylaminopropargyl deoxyuridine (DMAPdU) in place of thymidine and examined their ability to form intermolecular triple helices by thermal melting and DNase I footprinting studies. The results were compared with those for oligonucleotides containing 5-aminopropargyl-dU (APdU), 5-guanidinopropargyl-dU (GPdU) and 5-propynyl dU (PdU). We find that DMAPdU enhances triplex stability relative to T, though slightly less than the other analogues that bear positive charges (T << PdU < DMAPdU < APdU < GPdU). For oligonucleotides that contain multiple substitutions with DMAPdU dispersed residues are more effective than clustered combinations. DMAPdU will be especially useful as a nucleotide analogue as, unlike APdU and GPdU, the base does not require protection during oligonucleotide synthesis and it can therefore be used with other derivatives that require mild deprotection conditions. |
| spellingShingle | Rusling, D Peng, G Srinivasan, N Fox, K Brown, T DNA triplex formation with 5-dimethylaminopropargyl deoxyuridine. |
| title | DNA triplex formation with 5-dimethylaminopropargyl deoxyuridine. |
| title_full | DNA triplex formation with 5-dimethylaminopropargyl deoxyuridine. |
| title_fullStr | DNA triplex formation with 5-dimethylaminopropargyl deoxyuridine. |
| title_full_unstemmed | DNA triplex formation with 5-dimethylaminopropargyl deoxyuridine. |
| title_short | DNA triplex formation with 5-dimethylaminopropargyl deoxyuridine. |
| title_sort | dna triplex formation with 5 dimethylaminopropargyl deoxyuridine |
| work_keys_str_mv | AT ruslingd dnatriplexformationwith5dimethylaminopropargyldeoxyuridine AT pengg dnatriplexformationwith5dimethylaminopropargyldeoxyuridine AT srinivasann dnatriplexformationwith5dimethylaminopropargyldeoxyuridine AT foxk dnatriplexformationwith5dimethylaminopropargyldeoxyuridine AT brownt dnatriplexformationwith5dimethylaminopropargyldeoxyuridine |