"Split-and-Click" sgRNA
CRISPR-Cas9 gene editing is dependent on a programmable single guide RNA (sgRNA) that directs Cas9 endonuclease activity. This RNA is often generated by enzymatic reactions, however the process becomes time-consuming as the number of sgRNAs increases and does not allow the incorporation of chemical...
Main Authors: | Taemaitree, L, Shivalingam, A, El-Sagheer, AH, Brown, T |
---|---|
Format: | Journal article |
Language: | English |
Published: |
Springer Nature
2020
|
Similar Items
-
An artificial triazole backbone linkage provides a split-and-click strategy to bioactive chemically modified CRISPR sgRNA
by: Taemaitree, L, et al.
Published: (2019) -
An artificial triazole backbone linkage provides a split-and-click strategy to bioactive chemically modified CRISPR sgRNA
by: Lapatrada Taemaitree, et al.
Published: (2019-04-01) -
Sequence determinants of improved CRISPR sgRNA design
by: Xu, Han, et al.
Published: (2016) -
Using CRISPR-ERA Webserver for sgRNA Design
by: Honglei Liu, et al.
Published: (2017-09-01) -
DNA Nanocarriers for Delivery of sgRNA/Cas9 Ribonucleoprotein
by: Hanyin Zhu, et al.
Published: (2024-09-01)