"Split-and-Click" sgRNA

CRISPR-Cas9 gene editing is dependent on a programmable single guide RNA (sgRNA) that directs Cas9 endonuclease activity. This RNA is often generated by enzymatic reactions, however the process becomes time-consuming as the number of sgRNAs increases and does not allow the incorporation of chemical...

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Bibliographic Details
Main Authors: Taemaitree, L, Shivalingam, A, El-Sagheer, AH, Brown, T
Format: Journal article
Language:English
Published: Springer Nature 2020

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