Monitoring DNA replication in fission yeast by incorporation of 5-ethynyl-2'-deoxyuridine.

We report procedures to allow incorporation and detection of 5-ethynyl-2'-deoxyuridine (EdU) in fission yeast, a thymidine analogue which has some technical advantages over use of bromodeoxyuridine. Low concentrations of EdU (1 µM) are sufficient to allow detection of incorporation in cells exp...

Cur síos iomlán

Sonraí bibleagrafaíochta
Príomhchruthaitheoirí: Hua, H, Kearsey, S
Formáid: Journal article
Teanga:English
Foilsithe / Cruthaithe: 2011