Long-read sequencing for fast and robust identification of correct genome-edited alleles: PCR-based and Cas9 capture methods
<p><strong>Background</strong> Recent developments in CRISPR/Cas9 genome-editing tools have facilitated the introduction of precise alleles, including genetic intervals spanning several kilobases, directly into the embryo. However, the introduction of donor templates, via homology...
Main Authors: | , , , , , , , , , , , , , , , , , , , |
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Format: | Journal article |
Language: | English |
Published: |
Public Library of Science
2024
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