Long-read sequencing for fast and robust identification of correct genome-edited alleles: PCR-based and Cas9 capture methods

<p><strong>Background</strong> Recent developments in CRISPR/Cas9 genome-editing tools have facilitated the introduction of precise alleles, including genetic intervals spanning several kilobases, directly into the embryo. However, the introduction of donor templates, via homology...

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Bibliographic Details
Main Authors: McCabe, CV, Price, PD, Codner, GF, Allan, AJ, Caulder, A, Christou, S, Loeffler, J, Mackenzie, M, Malzer, E, Mianné, J, Nowicki, KJ, O'Neill, EJ, Pike, FJ, Hutchison, M, Petit-Demoulière, B, Stewart, ME, Gates, H, Wells, S, Sanderson, ND, Teboul, L
Format: Journal article
Language:English
Published: Public Library of Science 2024