The kinetic properties of various R258 mutants of deacetoxycephalosporin C synthase.

Site-directed mutagenesis was used to investigate the control of 2-oxoacid cosubstrate selectivity by deacetoxycephalosporin C synthase. The wild-type enzyme has a requirement for 2-oxoglutarate and cannot efficiently use hydrophobic 2-oxoacids (e.g. 2-oxohexanoic acid, 2-oxo-4-methyl-pentanoic acid...

সম্পূর্ণ বিবরণ

গ্রন্থ-পঞ্জীর বিবরন
প্রধান লেখক: Lee, H, Dai, Y, Shiau, C, Schofield, C, Lloyd, MD
বিন্যাস: Journal article
ভাষা:English
প্রকাশিত: 2003