The kinetic properties of various R258 mutants of deacetoxycephalosporin C synthase.

The kinetic properties of various R258 mutants of deacetoxycephalosporin C synthase.

Site-directed mutagenesis was used to investigate the control of 2-oxoacid cosubstrate selectivity by deacetoxycephalosporin C synthase. The wild-type enzyme has a requirement for 2-oxoglutarate and cannot efficiently use hydrophobic 2-oxoacids (e.g. 2-oxohexanoic acid, 2-oxo-4-methyl-pentanoic acid...

Szczegółowa specyfikacja

Opis bibliograficzny
Główni autorzy:	Lee, H, Dai, Y, Shiau, C, Schofield, C, Lloyd, MD
Format:	Journal article
Język:	English
Wydane:	2003

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