Stable end-sealed DNA as robust nano-rulers for in vivo single-molecule fluorescence

Single-molecule fluorescence and Förster resonance energy transfer (smFRET) are important tools for studying molecular heterogeneity, cellular organization, and protein structure in living cells. However, in vivo smFRET studies are still very challenging, and a standardized approach for robust in vi...

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Bibliographic Details
Main Authors: Plochowietz, A, El-Sagheer, A, Brown, T, Kapanidis, A
Format: Journal article
Published: Royal Society of Chemistry 2016