A flow cytometry-based strategy to identify and express IgM from VH1-69+ clonal peripheral B cells.
Pathologic rheumatoid factor (RF) levels are hallmarks of several human diseases. Production of monoclonal RF in vitro is essential for studies of the antigenic specificities of RF, as well as for a dissection of the mechanisms of aberrant RF+ B cell activation. We have expanded upon previous method...
Main Authors: | Charles, E, Orloff, M, Dustin, L |
---|---|
格式: | Journal article |
语言: | English |
出版: |
2011
|
相似书籍
-
Diversity and repertoire of IgW and IgM <it>VH </it>families in the newborn nurse shark
由: Dooley Helen, et al.
出版: (2004-05-01) -
In situ IgM production and clonal expansion of B-1 cells in peritoneal cavity promote elimination of C. albicans infection in IgH transgenic mice with VH derived from a natural antibody.
由: Meng Fu, et al.
出版: (2013-01-01) -
Correction: In Situ IgM Production and Clonal Expansion of B-1 Cells in Peritoneal Cavity Promote Elimination of C. albicans Infection in IgH Transgenic Mice with VH Derived from a Natural Antibody
出版: (2013-01-01) -
Correction: In situ IgM production and clonal expansion of B-1 cells in peritoneal cavity promote elimination of C. albicans infection in IgH transgenic mice with VH derived from a natural antibody.
由: PLOS ONE Staff
出版: (2015-01-01) -
Teleost fish mount complex clonal IgM and IgT responses in spleen upon systemic viral infection.
由: Rosario Castro, et al.
出版: (2013-01-01)