Generating subclones from large-insert genomic clones.
This unit provides a simple protocol for generating a partial-digest sublibrary of yeast DNA containing a YAC of interest. The starting material is high-molecular-weight chromosomal DNA embedded in agarose plugs. Many genome equivalents of these YAC subclones in bacteriophage or cosmid vectors can b...
| Main Authors: | , |
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| Format: | Journal article |
| Language: | English |
| Published: |
2001
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