Generating subclones from large-insert genomic clones.
This unit provides a simple protocol for generating a partial-digest sublibrary of yeast DNA containing a YAC of interest. The starting material is high-molecular-weight chromosomal DNA embedded in agarose plugs. Many genome equivalents of these YAC subclones in bacteriophage or cosmid vectors can b...
| Huvudupphovsmän: | , |
|---|---|
| Materialtyp: | Journal article |
| Språk: | English |
| Publicerad: |
2001
|