Cis-acting sequences regulating expression of the human alpha-globin cluster lie within constitutively open chromatin.
Current models suggest that tissue-specific genes are arranged in discrete, independently controlled segments of chromatin referred to as regulatory domains. Transition from a closed to open chromatin structure may be an important step in the regulation of gene expression. To determine whether the h...
| Main Authors: | , , , , , |
|---|---|
| Format: | Journal article |
| Language: | English |
| Published: |
1992
|
| _version_ | 1826304621473169408 |
|---|---|
| author | Vyas, P Vickers, M Simmons, D Ayyub, H Craddock, C Higgs, D |
| author_facet | Vyas, P Vickers, M Simmons, D Ayyub, H Craddock, C Higgs, D |
| author_sort | Vyas, P |
| collection | OXFORD |
| description | Current models suggest that tissue-specific genes are arranged in discrete, independently controlled segments of chromatin referred to as regulatory domains. Transition from a closed to open chromatin structure may be an important step in the regulation of gene expression. To determine whether the human alpha-globin cluster, like the beta-globin cluster, lies within a discrete, erythroid-specific domain, we have examined the long-range genomic organization and chromatin structure around this region. The alpha genes lie adjacent to at least four widely expressed genes. The major alpha-globin regulatory element lies 40 kb away from the cluster within an intron of one of these genes. Therefore, unlike the beta cluster, cis-acting sequences controlling alpha gene expression are dispersed within a region of chromatin that is open in both erythroid and nonerythroid cells. This implies a difference in the hierarchical control of alpha- and beta-globin expression. |
| first_indexed | 2024-03-07T06:20:36Z |
| format | Journal article |
| id | oxford-uuid:f2964de8-c58d-43c3-a832-b856e407e6c7 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T06:20:36Z |
| publishDate | 1992 |
| record_format | dspace |
| spelling | oxford-uuid:f2964de8-c58d-43c3-a832-b856e407e6c72022-03-27T12:04:58ZCis-acting sequences regulating expression of the human alpha-globin cluster lie within constitutively open chromatin.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:f2964de8-c58d-43c3-a832-b856e407e6c7EnglishSymplectic Elements at Oxford1992Vyas, PVickers, MSimmons, DAyyub, HCraddock, CHiggs, DCurrent models suggest that tissue-specific genes are arranged in discrete, independently controlled segments of chromatin referred to as regulatory domains. Transition from a closed to open chromatin structure may be an important step in the regulation of gene expression. To determine whether the human alpha-globin cluster, like the beta-globin cluster, lies within a discrete, erythroid-specific domain, we have examined the long-range genomic organization and chromatin structure around this region. The alpha genes lie adjacent to at least four widely expressed genes. The major alpha-globin regulatory element lies 40 kb away from the cluster within an intron of one of these genes. Therefore, unlike the beta cluster, cis-acting sequences controlling alpha gene expression are dispersed within a region of chromatin that is open in both erythroid and nonerythroid cells. This implies a difference in the hierarchical control of alpha- and beta-globin expression. |
| spellingShingle | Vyas, P Vickers, M Simmons, D Ayyub, H Craddock, C Higgs, D Cis-acting sequences regulating expression of the human alpha-globin cluster lie within constitutively open chromatin. |
| title | Cis-acting sequences regulating expression of the human alpha-globin cluster lie within constitutively open chromatin. |
| title_full | Cis-acting sequences regulating expression of the human alpha-globin cluster lie within constitutively open chromatin. |
| title_fullStr | Cis-acting sequences regulating expression of the human alpha-globin cluster lie within constitutively open chromatin. |
| title_full_unstemmed | Cis-acting sequences regulating expression of the human alpha-globin cluster lie within constitutively open chromatin. |
| title_short | Cis-acting sequences regulating expression of the human alpha-globin cluster lie within constitutively open chromatin. |
| title_sort | cis acting sequences regulating expression of the human alpha globin cluster lie within constitutively open chromatin |
| work_keys_str_mv | AT vyasp cisactingsequencesregulatingexpressionofthehumanalphaglobinclusterliewithinconstitutivelyopenchromatin AT vickersm cisactingsequencesregulatingexpressionofthehumanalphaglobinclusterliewithinconstitutivelyopenchromatin AT simmonsd cisactingsequencesregulatingexpressionofthehumanalphaglobinclusterliewithinconstitutivelyopenchromatin AT ayyubh cisactingsequencesregulatingexpressionofthehumanalphaglobinclusterliewithinconstitutivelyopenchromatin AT craddockc cisactingsequencesregulatingexpressionofthehumanalphaglobinclusterliewithinconstitutivelyopenchromatin AT higgsd cisactingsequencesregulatingexpressionofthehumanalphaglobinclusterliewithinconstitutivelyopenchromatin |