Selection of functional single domain antibody fragments for interfering with protein-protein interactions inside cells: a "one plasmid" mammalian two-hybrid system.

Selection of functional single domain antibody fragments for interfering with protein-protein interactions inside cells: a "one plasmid" mammalian two-hybrid system.

As a complement to the intracellular antibody capture method to isolate intracellular single domain antibody fragments (iDabs) from high diverse libraries, we describe here a simple mammalian two-hybrid (M2H) protocol using a "bait-prey hybrid single plasmid" to assess those interfering iD...

पूर्ण विवरण

ग्रंथसूची विवरण
मुख्य लेखकों:	Tanaka, T, Rabbitts, T
स्वरूप:	Book section
प्रकाशित:	2012

समान संसाधन

Single Domain Antibody Fragments as Drug Surrogates Targeting Protein–Protein Interactions inside Cells
द्वारा: Terence H. Rabbitts, और अन्य
प्रकाशित: (2013-05-01)

Interfering with protein-protein interactions: potential for cancer therapy.
द्वारा: Tanaka, T, और अन्य
प्रकाशित: (2008)

Protocol for the selection of single-domain antibody fragments by third generation intracellular antibody capture.
द्वारा: Tanaka, T, और अन्य
प्रकाशित: (2010)

Selection of complementary single-variable domains for building monoclonal antibodies to native proteins.
द्वारा: Tanaka, T, और अन्य
प्रकाशित: (2009)

The selection of single-domain antibody fragments (iDAbs) by intracellular antibody capture in yeast
द्वारा: Rabbitts, T, और अन्य
प्रकाशित: (2018)

Tumour prevention by a single antibody domain targeting the interaction of signal transduction proteins with RAS.
द्वारा: Tanaka, T, और अन्य
प्रकाशित: (2007)

Single domain intracellular antibodies: a minimal fragment for direct in vivo selection of antigen-specific intrabodies.
द्वारा: Tanaka, T, और अन्य
प्रकाशित: (2003)

Isolation of antigen-specific intracellular antibody fragments as single chain Fv for use in mammalian cells.
द्वारा: Tse, E, और अन्य
प्रकाशित: (2002)

Intracellular antibody capture (IAC) methods for single domain antibodies.
द्वारा: Tanaka, T, और अन्य
प्रकाशित: (2012)

Functional intracellular antibody fragments do not require invariant intra-domain disulfide bonds.
द्वारा: Tanaka, T, और अन्य
प्रकाशित: (2008)

Single domain intracellular antibodies from diverse libraries: emphasizing dual functions of LMO2 protein interactions using a single VH domain.
द्वारा: Tanaka, T, और अन्य
प्रकाशित: (2011)

Interfering with RAS-effector protein interactions prevent RAS-dependent tumour initiation and causes stop-start control of cancer growth.
द्वारा: Tanaka, T, और अन्य
प्रकाशित: (2010)

A plasmid-based system for expressing small interfering RNA libraries in mammalian cells
द्वारा: Moon Randall T, और अन्य
प्रकाशित: (2004-04-01)

Design of a single plasmid-based modified yeast one-hybrid system for investigation of in vivo protein-protein and protein-DNA interactions
द्वारा: Gang Chen, और अन्य
प्रकाशित: (2008-09-01)

An Inside Job: Applications of Intracellular Single Domain Antibodies
द्वारा: Eline Soetens, और अन्य
प्रकाशित: (2020-12-01)

Regulation of protein function by interfering protein species
द्वारा: Graeff Moritz, और अन्य
प्रकाशित: (2012-02-01)

Protein Expression Both in Mammalian Cell Lines and in Yeast Pichia pastoris Using a Single Expression Plasmid
द्वारा: Zhong Liu, और अन्य
प्रकाशित: (1998-02-01)

Biotechnological applications of recombinant single-domain antibody fragments
द्वारा: de Marco Ario
प्रकाशित: (2011-06-01)

Intracellular antibody capture: A molecular biology approach to inhibitors of protein-protein interactions.
द्वारा: Zhang, J, और अन्य
प्रकाशित: (2014)

Single-domain antibody-based protein degrader for synucleinopathies
द्वारा: Yixiang Jiang, और अन्य
प्रकाशित: (2024-05-01)

Single Framework Recombinant Antibody Fragments Designed for Protein Chip Applications
द्वारा: Cornelia Steinhauer, और अन्य
प्रकाशित: (2002-12-01)

Small molecule inhibitors of RAS-effector protein interactions derived using an intracellular antibody fragment
द्वारा: Quevedo, C, और अन्य
प्रकाशित: (2018)

Intracellular antibody capture technology: application to selection of intracellular antibodies recognising the BCR-ABL oncogenic protein.
द्वारा: Tse, E, और अन्य
प्रकाशित: (2002)

Synthetic single domain antibodies for the conformational trapping of membrane proteins
द्वारा: Iwan Zimmermann, और अन्य
प्रकाशित: (2018-05-01)

Overcoming Obstacles in DNA Sequencing of Expression Plasmids for Short Interfering RNAs
द्वारा: Daniel C. Ducat, और अन्य
प्रकाशित: (2003-06-01)

Facilitating plasmid nuclear delivery by interfering with the selective nuclear pore barrier
द्वारा: Ihab Azzam, और अन्य
प्रकाशित: (2019-09-01)

Endogenous Human Proteins Interfering with Amyloid Formation
द्वारा: Anna L. Gharibyan, और अन्य
प्रकाशित: (2022-03-01)

Single-domain antibody fragments derived from heavy-chain antibodies: a review
द्वारा: L. Eyer, और अन्य
प्रकाशित: (2012-09-01)

Single domain Camelid antibody fragments for molecular imaging and therapy of cancer
द्वारा: Shulin Li, और अन्य
प्रकाशित: (2023-09-01)

Small molecule inhibitors of RAS-effector protein interactions derived using an intracellular antibody fragment
द्वारा: Camilo E. Quevedo, और अन्य
प्रकाशित: (2018-08-01)

The Lymphocytic Choriomeningitis Virus Matrix Protein PPXY Late Domain Drives the Production of Defective Interfering Particles.
द्वारा: Christopher M Ziegler, और अन्य
प्रकाशित: (2016-03-01)

An antibody inhibitor of the LMO2-protein complex blocks its normal and tumorigenic functions.
द्वारा: Nam, C, और अन्य
प्रकाशित: (2008)

Converting monoclonal antibodies into Fab fragments for transient expression in mammalian cells.
द्वारा: Nettleship, J, और अन्य
प्रकाशित: (2012)

The production of antibody fragments and antibody fusion proteins by yeasts and filamentous fungi
द्वारा: van den Hondel Cees AMJJ, और अन्य
प्रकाशित: (2003-01-01)

In silico Approaches for the Design and Optimization of Interfering Peptides Against Protein–Protein Interactions
द्वारा: Zahra Sadat Hashemi, और अन्य
प्रकाशित: (2021-04-01)

Antibody Fragments and Their Purification by Protein L Affinity Chromatography
द्वारा: Gustav Rodrigo, और अन्य
प्रकाशित: (2015-09-01)

Cancer cell killing by target antigen engagement with engineered complementary intracellular antibody single domains fused to pro-caspase3
द्वारा: Chambers, J, और अन्य
प्रकाशित: (2019)

Building Protein Domain Based Composite Biobricks for Mammalian Expression Systems
द्वारा: Balint, Balint L, और अन्य
प्रकाशित: (2010)

Selection of intracellular single-domain antibodies targeting the HIV-1 Vpr protein by cytoplasmic yeast two-hybrid system.
द्वारा: Julie Matz, और अन्य
प्रकाशित: (2014-01-01)

A platform-agnostic, function first-based antibody discovery strategy using plasmid-free mammalian expression of antibodies
द्वारा: Ruijun Zhang, और अन्य
प्रकाशित: (2021-01-01)