Purification of DNA repair protein complexes from mammalian cells

Cells possess multiple DNA repair pathways to tackle a variety of DNA lesions. Often, DNA repair proteins function as large protein complexes. Here, we describe a protocol to purify DNA repair protein complexes from nuclei of mammalian cells. The method permits purification of protein complexes cont...

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Bibliographic Details
Main Authors: Liang, C-C, Cohn, MA
Format: Journal article
Language:English
Published: Elsevier 2021